ArticleBiomicrofluidics2013
Enrichment of live unlabelled cardiomyocytes from heterogeneous cell populations using manipulation of cell settling velocity by magnetic field.
Article in Biomicrofluidics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- A pump-free microfluidic device for fast magnetic labeling of ischemic stroke biomarkers.Analytical and bioanalytical chemistry · 2022Article
- An Early Stage Researcher's Primer on Systems Medicine Terminology.Network and systems medicine · 2021Review
- Intrinsically Magnetic Cells: A Review on Their Natural Occurrence and Synthetic Generation.Frontiers in bioengineering and biotechnology · 2020Review
- Current Strategies and Challenges for Purification of Cardiomyocytes Derived from Human Pluripotent Stem Cells.Theranostics · 2017Review
- On-Chip Magnetic Bead Manipulation and Detection Using a Magnetoresistive Sensor-Based Micro-Chip: Design Considerations and Experimental Characterization.Sensors (Basel, Switzerland) · 2016Article
- Microfluidic Sample Preparation for Single Cell Analysis.Analytical chemistry · 2016Review
- Fundamentals and application of magnetic particles in cell isolation and enrichment: a review.Reports on progress in physics. Physical Society (Great Britain) · 2015Review
- Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip.Biomicrofluidics · 2014Article
- Microfluidic immunomagnetic cell separation using integrated permanent micromagnets.Biomicrofluidics · 2013Article
- On-chip magnetophoretic isolation of CD4 + T cells from blood.Biomicrofluidics · 2013Article
- On-chip microfluidic biosensor using superparamagnetic microparticles.Biomicrofluidics · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The majority of available cardiomyocyte markers are intercellular proteins, limiting our ability to enrich live cardiomyocytes from heterogeneous cell preparations in the absence of genetic labeling. Here, we describe enrichment of live cardiomyocytes from the hearts of adult mice in a label-free microfluidic approach. The separation device consisted of a vertical column (15 mm long, 700 μm diameter), placed between permanent magnets resulting in a field strength of 1.23 T. To concentrate the field at the column wall, the column was wrapped with 69 μm diameter nickel wire. Before passing the cells through the column, the cardiomyocytes in the cell suspension had been rendered paramagnetic by treatment of the adult mouse heart cell preparation with sodium nitrite (2.5 mM) for 20 min on ice. The cell suspension was loaded into the vertical column from the top and upon settling, the non-myocytes were removed by the upward flow from the column. The cardiomyocytes were then collected from the column by applying a higher flow rate (144 μl/min). We found that by applying a separation flow rate of 4.2 μl/min in the first step, we can enrich live adult cardiomyocytes to 93% ± 2% in a label-free manner. The cardiomyocytes maintained viability immediately after separation and upon 24 h in culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.