ArticleMolecular biology of the cell2014
A microscopy-based screen employing multiplex genome sequencing identifies cargo-specific requirements for dynein velocity.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Endosome motility controls light-responsive reproductive development and secondary metabolite production in Aspergillus.Current biology : CB · 2026Article
- Acyl-CoA-binding protein AcbdA is required for peroxisome hitchhiking on early endosomes inMolecular biology of the cell · 2025Article
- Aspergillus SUMOylation mutants exhibit chromosome segregation defects including chromatin bridges.Genetics · 2023Article
- Woronin body hitchhiking on early endosomes is dispensable for septal localization inMolecular biology of the cell · 2023Article
- Woronin bodies move dynamically and bidirectionally by hitchhiking on early endosomes inbioRxiv : the preprint server for biology · 2023Article
- Optimizing microtubule arrangements for rapid cargo capture.Biophysical journal · 2021Article
- Dynein activation in vivo is regulated by the nucleotide states of its AAA3 domain.Current biology : CB · 2021Article
- PxdA interacts with the DipA phosphatase to regulate peroxisome hitchhiking on early endosomes.Molecular biology of the cell · 2021Article
- Nuclear movement in fungi.Seminars in cell & developmental biology · 2018Review
- Peroxisomes move by hitchhiking on early endosomes using the novel linker protein PxdA.The Journal of cell biology · 2016Article
- The Adenylate-Forming Enzymes AfeA and TmpB Are Involved in Aspergillus nidulans Self-Communication during Asexual Development.Frontiers in microbiology · 2016Article
- Yeast GSK-3 kinase regulates astral microtubule function through phosphorylation of the microtubule-stabilizing kinesin Kip2.Journal of cell science · 2015Article
- Cytoplasmic dynein and early endosome transport.Cellular and molecular life sciences : CMLS · 2015Review
- ER network homeostasis is critical for plant endosome streaming and endocytosis.Cell discovery · 2015Article
- Characterization of the mutagenic spectrum of 4-nitroquinoline 1-oxide (4-NQO) in Aspergillus nidulans by whole genome sequencing.G3 (Bethesda, Md.) · 2014Article
- FHIP and FTS proteins are critical for dynein-mediated transport of early endosomes in Aspergillus.Molecular biology of the cell · 2014Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The timely delivery of membranous organelles and macromolecules to specific locations within the majority of eukaryotic cells depends on microtubule-based transport. Here we describe a screening method to identify mutations that have a critical effect on intracellular transport and its regulation using mutagenesis, multicolor-fluorescence microscopy, and multiplex genome sequencing. This screen exploits the filamentous fungus Aspergillus nidulans, which has many of the advantages of yeast molecular genetics but uses long-range microtubule-based transport in a manner more similar to metazoan cells. Using this method, we identified seven mutants that represent novel alleles of components of the intracellular transport machinery: specifically, kinesin-1, cytoplasmic dynein, and the dynein regulators Lis1 and dynactin. The two dynein mutations identified in our screen map to dynein's AAA+ catalytic core. Single-molecule studies reveal that both mutations reduce dynein's velocity in vitro. In vivo these mutants severely impair the distribution and velocity of endosomes, a known dynein cargo. In contrast, another dynein cargo, the nucleus, is positioned normally in these mutants. These results reveal that different dynein functions have distinct stringencies for motor performance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.