ArticleJournal of molecular endocrinology2014
PCP4: a regulator of aldosterone synthesis in human adrenocortical tissues.
Article in Journal of molecular endocrinology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Comparative Proteomic Profiling of Adrenocortical Neoplasia Using Mass Spectrometry.Journal of proteome research · 2025Article
- Primary aldosteronism: molecular medicine meets public health.Nature reviews. Nephrology · 2023Review
- Novel regulation mechanism of adrenal cortisol and DHEA biosynthesis via the endogen ERAD inhibitor small VCP-interacting protein.Scientific reports · 2022Article
- Molecular Mechanisms of Functional Adrenocortical Adenoma and Carcinoma: Genetic Characterization and Intracellular Signaling Pathway.Biomedicines · 2021Review
- The landscape of molecular mechanism for aldosterone production in aldosterone-producing adenoma.Endocrine journal · 2020Review
- Novel molecular mechanisms in the inhibition of adrenal aldosterone synthesis: Action of tolvaptan via vasopressin VBritish journal of pharmacology · 2019Article
- Tumor Cell Subtypes Based on the Intracellular Hormonal Activity in KCNJ5-Mutated Aldosterone-Producing Adenoma.Hypertension (Dallas, Tex. : 1979) · 2018Article
- PCP4/PEP19 upregulates aromatase gene expression via CYP19A1 promoter I.1 in human breast cancer SK-BR-3 cells.Oncotarget · 2018Article
- Upregulation of PCP4 in human aldosterone-producing adenomas fosters human adrenocortical tumor cell growth via AKT and AMPK pathway.International journal of clinical and experimental pathology · 2018Article
- High glucose stimulates expression of aldosterone synthase (FEBS open bio · 2017Article
- PCP4/PEP19 promotes migration, invasion and adhesion in human breast cancer MCF-7 and T47D cells.Oncotarget · 2016Article
- 3β-Hydroxysteroid dehydrogenase isoforms in human aldosterone-producing adenoma.Molecular and cellular endocrinology · 2015Article
- Pre-B Lymphocyte Protein 3 (VPREB3) Expression in the Adrenal Cortex: Precedent for non-Immunological Roles in Normal and Neoplastic Human Tissues.Endocrine pathology · 2015Article
- DACH1, a zona glomerulosa selective gene in the human adrenal, activates transforming growth factor-β signaling and suppresses aldosterone secretion.Hypertension (Dallas, Tex. : 1979) · 2015Article
- Anti-apoptotic effects of PCP4/PEP19 in human breast cancer cell lines: a novel oncotarget.Oncotarget · 2014Article
- Adrenal CYP11B1/2 expression in primary aldosteronism: immunohistochemical analysis using novel monoclonal antibodies.Molecular and cellular endocrinology · 2014Article
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
Purkinje cell protein 4 (PCP4) is a calmodulin (CaM)-binding protein that accelerates calcium association and dissociation with CaM. It has been previously detected in aldosterone-producing adenomas (APA), but details on its expression and function in adrenocortical tissues have remained unknown. Therefore, we performed the immunohistochemical analysis of PCP4 in the following tissues: normal adrenal (NA; n=15), APA (n=15), cortisol-producing adenomas (n=15), and idiopathic hyperaldosteronism cases (IHA; n=5). APA samples (n=45) were also submitted to quantitative RT-PCR of PCP4, CYP11B1, and CYP11B2, as well as DNA sequencing for KCNJ5 mutations. Transient transfection analysis using PCP4 siRNA was also performed in H295R adrenocortical carcinoma cells, following ELISA analysis, and CYP11B2 luciferase assays were also performed after PCP4 vector transfection in order to study the regulation of PCP4 protein expression. In our findings, PCP4 immunoreactivity was predominantly detected in APA and in the zona glomerulosa of NA and IHA. In APA, the mRNA levels of PCP4 were significantly correlated with those of CYP11B2 (P<0.0001) and were significantly higher in cases with KCNJ5 mutation than WT (P=0.005). Following PCP4 vector transfection, CYP11B2 luciferase reporter activity was significantly higher than controls in the presence of angiotensin-II. Knockdown of PCP4 resulted in a significant decrease in CYP11B2 mRNA levels (P=0.012) and aldosterone production (P=0.011). Our results indicate that PCP4 is a regulator of aldosterone production in normal, hyperplastic, and neoplastic human adrenocortical cells.
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