ArticleThe Journal of biological chemistry2014
Evolution of mammalian Opn5 as a specialized UV-absorbing pigment by a single amino acid mutation.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 98 citations in OpenAlex.
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- Prolonged Light Exposure Induces Long-Lasting Retinal Wave Plasticity via Retrograde Melanopsin-Dopamine Signaling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Non-visual opsins and their role in circadian photoentrainment.Eye and vision (London, England) · 2026Review
- The physiological functions of OPN5m.Eye and vision (London, England) · 2025Review
- Non-visual photoreceptive brain specification in sea urchin larvae.Nature communications · 2025Article
- Discovery and design of photocyclic animal opsins: potential application to gene therapy from non-visual opsin research.Eye and vision (London, England) · 2025Review
- A key spectral tuning site of UV-sensitive vertebrate non-visual opsin Opn5.Cellular and molecular life sciences : CMLS · 2025Article
- Development of visible light-sensitive human neuropsin (OPN5) via single amino acid substitution.FEBS letters · 2025Article
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- Expression of opsin and visual cycle-related enzymes in fetal rat skin keratinocytes and cellular response to blue light.Biochemistry and biophysics reports · 2024Article
- A bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits.Nature methods · 2024Article
- Functional characterization of four opsins and two G alpha subtypes co-expressed in the molluscan rhabdomeric photoreceptor.BMC biology · 2023Article
- A bistable inhibitory OptoGPCR for multiplexed optogenetic control of neural circuits.bioRxiv : the preprint server for biology · 2023Article
- Photobiomodulation at molecular, cellular, and systemic levels.Lasers in medical science · 2023Review
- IMI 2023 Digest.Investigative ophthalmology & visual science · 2023Article
- Photons guided by axons may enable backpropagation-based learning in the brain.Scientific reports · 2022Article
- Shining more light on G protein signalling modules: a novel optogenetic tool for Gq activation.Science China. Life sciences · 2022Article
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Opn5 is one of the recently identified opsin groups that is responsible for nonvisual photoreception in animals. We previously showed that a chicken homolog of mammalian Opn5 (Opn5m) is a Gi-coupled UV sensor having molecular properties typical of bistable pigments. Here we demonstrated that mammalian Opn5m evolved to be a more specialized photosensor by losing one of the characteristics of bistable pigments, direct binding of all-trans-retinal. We first confirmed that Opn5m proteins in zebrafish, Xenopus tropicalis, mouse, and human are also UV-sensitive pigments. Then we found that only mammalian Opn5m proteins lack the ability to directly bind all-trans-retinal. Mutational analysis showed that these characteristics were acquired by a single amino acid replacement at position 168. By comparing the expression patterns of Opn5m between mammals and chicken, we found that, like chicken Opn5m, mammalian Opn5m was localized in the ganglion cell layer and inner nuclear layer of the retina. However, the mouse and primate (common marmoset) opsins were distributed not in the posterior hypothalamus (including the region along the third ventricle) where chicken Opn5m is localized, but in the preoptic hypothalamus. Interestingly, RPE65, an essential enzyme for forming 11-cis-retinal in the visual cycle is expressed near the preoptic hypothalamus of the mouse and common marmoset brain but not near the region of the chicken brain where chicken Opn5m is expressed. Therefore, mammalian Opn5m may work exclusively as a short wavelength sensor in the brain as well as in the retina with the assistance of an 11-cis-retinal-supplying system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.