ArticleMemorias do Instituto Oswaldo Cruz2013
A conventional polymerase chain reaction-based method for the diagnosis of human schistosomiasis in stool samples from individuals in a low-endemicity area.
Article in Memorias do Instituto Oswaldo Cruz, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Accuracy Study of Kato-Katz and Helmintex Methods for Diagnosis of Schistosomiasis Mansoni in a Moderate Endemicity Area in Sergipe, Northeastern Brazil.Diagnostics (Basel, Switzerland) · 2023Article
- Assessment of the accuracy of 11 different diagnostic tests for the detection ofFrontiers in microbiology · 2022Article
- Accuracy of real-time polymerase chain reaction to detectParasitology · 2020Article
- A Real-Time PCR Assay for the Diagnosis of Intestinal Schistosomiasis and Cure Assessment After the Treatment of Individuals With Low Parasite Burden.Frontiers in immunology · 2020Article
- Diagnosis of Schistosoma mansoni infections: what are the choices in Brazilian low-endemic areas?Memorias do Instituto Oswaldo Cruz · 2019Review
- Performance of an Ultra-Sensitive Assay Targeting the Circulating Anodic Antigen (CAA) for Detection ofFrontiers in immunology · 2019Article
- Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA.BMC research notes · 2018Article
- A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples.PLoS neglected tropical diseases · 2018Article
- Nucleic acid detection in the diagnosis and prevention of schistosomiasis.Infectious diseases of poverty · 2016Review
- Advances in the Diagnosis of Human Schistosomiasis.Clinical microbiology reviews · 2015Review
- Evaluation of the sensitivity of IgG and IgM ELISA in detecting Schistosoma mansoni infections in a low endemicity setting.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to evaluate the efficacy of a polymerase chain reaction (PCR)-based method to detect Schistosoma mansoni DNA in stool samples from individuals living in a low-endemicity area in Brazil. Of the 125 initial stool samples, 80 were ELISA reactive and eggs were identified in 19 of the samples by parasitological examination. For the PCR evaluations, 56 stool samples were selected and divided into five groups. Groups I-IV were scored negative for S. mansoni eggs by parasitological examination. Groups I and II were ELISA reactive, whereas Groups III and IV were ELISA nonreactive. Groups II and III were positive for other intestinal parasites. PCR testing scored eight samples as positive from these four groups. Group V represented the S. mansoni -positive group and it included ELISA-reactive samples that were scored positive for S. mansoni by one or more parasitological examinations (6/19 were positive by Kato-Katz method, 9/17 by saline gradient and 10/13 by Helmintex®). PCR scored 13 of these 19 samples as positive for S. mansoni . We conclude that while none of these methods yielded 100% sensitivity, a combination of techniques should be effective for improving the detection of S. mansoni infection in low-endemicity areas.
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