ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2014
Na+/H+ and Na+/NH+4 activities of zebrafish NHE3b expressed in Xenopus oocytes.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
- Ion uptake in naturally acidic water.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2024Review
- Molecular Physiological Evidence for the Role of NaInternational journal of molecular sciences · 2023Article
- Acute Stress in Lesser-Spotted Catshark (Animals : an open access journal from MDPI · 2022Article
- Mechanisms of NaFrontiers in physiology · 2022Review
- Teleostean fishes may have developed an efficient NaFrontiers in physiology · 2022Article
- Cortisol and glucocorticoid receptor 2 regulate acid secretion in medaka (Oryzias latipes) larvae.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2021Article
- Rainbow Trout (International journal of molecular sciences · 2021Article
- Expression of ion transport genes in ionocytes isolated from larval zebrafish (American journal of physiology. Regulatory, integrative and comparative physiology · 2020Article
- NaThe Journal of physiology · 2017Article
- A role for sodium-chloride cotransporters in the rapid regulation of ion uptake following acute environmental acidosis: new insights from the zebrafish model.American journal of physiology. Cell physiology · 2016Article
- An emerging role for gasotransmitters in the control of breathing and ionic regulation in fish.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2016Review
- Transport proteins NHA1 and NHA2 are essential for survival, but have distinct transport modalities.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Hydrogen sulfide inhibits Na+ uptake in larval zebrafish, Danio rerio.Pflugers Archiv : European journal of physiology · 2015Article
- Mudskipper genomes provide insights into the terrestrial adaptation of amphibious fishes.Nature communications · 2014Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Zebrafish Na(+)/H(+) exchanger 3b (zNHE3b) is highly expressed in the apical membrane of ionocytes where Na(+) is absorbed from ion-poor fresh water against a concentration gradient. Much in vivo data indicated that zNHE3b is involved in Na(+) absorption but not leakage. However, zNHE3b-mediated Na(+) absorption has not been thermodynamically explained, and zNHE3b activity has not been measured. To address this issue, we overexpressed zNHE3b in Xenopus oocytes and characterized its activity by electrophysiology. Exposure of zNHE3b oocytes to Na(+)-free media resulted in significant decrease in intracellular pH (pH(i)) and intracellular Na(+) activity (aNa(i)). aNa(i) increased significantly when the cytoplasm was acidified by media containing CO₂-HCO₃(-) or butyrate. Activity of zNHE3b was inhibited by amiloride or 5-ethylisopropyl amiloride (EIPA). Although the activity was accompanied by a large hyperpolarization of ∼50 mV, voltage-clamp experiments showed that Na(+)/H(+) exchange activity of zNHE3b is electroneutral. Exposure of zNHE3b oocytes to medium containing NH₃/NH₄(+) resulted in significant decreases in pH(i) and aNa(i) and significant increase in intracellular NH₄(+) activity, indicating that zNHE3b mediates the Na(+)/NH₄(+) exchange. In low-Na(+) (0.5 mM) media, zNHE3b oocytes maintained aNa(i) of 1.3 mM, and Na(+)-influx was observed when pHi was decreased by media containing CO₂-HCO₃(-) or butyrate. These results provide thermodynamic evidence that zNHE3b mediates Na(+) absorption from ion-poor fresh water by its Na(+)/H(+) and Na(+)/NH₄(+) exchange activities.
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