ArticleEMBO reports2014
Structured mRNA induces the ribosome into a hyper-rotated state.
Article in EMBO reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Ribosome-induced mRNA pseudoknot interactions visualized by DMS MaP-Seq.bioRxiv : the preprint server for biology · 2025Article
- The influence of downstream structured elements within mRNA on the dynamics of intersubunit rotation in ribosomes.RNA (New York, N.Y.) · 2025Article
- Comparing FRET Pairs that Report on Intersubunit Rotation in Bacterial Ribosomes.Journal of molecular biology · 2023Article
- Comparing FRET pairs that report on intersubunit rotation in bacterial ribosomes.bioRxiv : the preprint server for biology · 2023Article
- Ribosomal RNA 2'-Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Specific length and structure rather than high thermodynamic stability enable regulatory mRNA stem-loops to pause translation.Nature communications · 2022Article
- Structural and molecular basis for Cardiovirus 2A protein as a viral gene expression switch.Nature communications · 2021Article
- Modulation of Viral Programmed Ribosomal Frameshifting and Stop Codon Readthrough by the Host Restriction Factor Shiftless.Viruses · 2021Article
- Programmed -1 ribosomal frameshifting from the perspective of the conformational dynamics of mRNA and ribosomes.Computational and structural biotechnology journal · 2021Review
- Article
- Translational recoding: canonical translation mechanisms reinterpreted.Nucleic acids research · 2020Review
- An in vitro single-molecule assay for eukaryotic cap-dependent translation initiation kinetics.Nucleic acids research · 2020Article
- The energy landscape of -1 ribosomal frameshifting.Science advances · 2020Article
- Ribosome collisions alter frameshifting at translational reprogramming motifs in bacterial mRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- EF-G-induced ribosome sliding along the noncoding mRNA.Science advances · 2019Article
- A tandem active site model for the ribosomal helicase.FEBS letters · 2019Article
- Article
- Alternative Mode of E-Site tRNA Binding in the Presence of a Downstream mRNA Stem Loop at the Entrance Channel.Structure (London, England : 1993) · 2018Article
- EF-G catalyzed translocation dynamics in the presence of ribosomal frameshifting stimulatory signals.Nucleic acids research · 2017Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During protein synthesis, mRNA and tRNA are moved through the ribosome by the process of translocation. The small diameter of the mRNA entrance tunnel only permits unstructured mRNA to pass through. However, there are structured elements within mRNA that present a barrier for translocation that must be unwound. The ribosome has been shown to unwind RNA in the absence of additional factors, but the mechanism remains unclear. Here, we show using single molecule Förster resonance energy transfer and small angle X-ray scattering experiments a new global conformational state of the ribosome. In the presence of the frameshift inducing dnaX hairpin, the ribosomal subunits are driven into a hyper-rotated state and the L1 stalk is predominantly in an open conformation. This previously unobserved conformational state provides structural insight into the helicase activity of the ribosome and may have important implications for understanding the mechanism of reading frame maintenance.
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