ArticleMicrobial cell factories2014
A single vector-based strategy for marker-less gene replacement in Synechocystis sp. PCC 6803.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Photosystem I-independent oxygenic photosynthesis in cyanobacteria.Nature communications · 2026Article
- A carbonic anhydrase-based nanogel for cyanobacterial growth enhancement.Materials today. Bio · 2025Article
- CurT/CURT1 proteins are involved in cell and chloroplast division coordination of cyanobacteria and green algae.Nature communications · 2025Article
- Pioneering precision in markerless strain development for Synechococcus sp. PCC 7002.Microbial cell factories · 2024Article
- Extracellular CahB1 from Sodalinema gerasimenkoae IPPAS B-353 Acts as a Functional Carboxysomal β-Carbonic Anhydrase in Synechocystis sp. PCC6803Plants (Basel, Switzerland) · 2023Article
- Metabolic engineering ofMetabolic engineering communications · 2021Article
- Enhancing photosynthesis at high light levels by adaptive laboratory evolution.Nature plants · 2021Article
- Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.Genes · 2021Review
- Engineering Improved Photosynthesis in the Era of Synthetic Biology.Plant communications · 2020Review
- Evidence that cyanobacterial Sll1217 functions analogously to PGRL1 in enhancing PGR5-dependent cyclic electron flow.Nature communications · 2019Article
- Genetic Engineering, Synthetic Biology and the Light Reactions of Photosynthesis.Plant physiology · 2019Review
- New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering.Frontiers in bioengineering and biotechnology · 2019Review
- Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803.Microbial cell factories · 2018Article
- Enhancing (crop) plant photosynthesis by introducing novel genetic diversity.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Article
- Computational metabolic engineering strategies for growth-coupled biofuel production byMetabolic engineering communications · 2016Article
- Cyanobacteria as Chassis for Industrial Biotechnology: Progress and Prospects.Life (Basel, Switzerland) · 2016Review
- Photosystem II Assembly from Scratch.Frontiers in plant science · 2015Article
- Cyanobacteria as cell factories to produce plant secondary metabolites.Frontiers in bioengineering and biotechnology · 2015Review
- Synechocystis: Not Just a Plug-Bug for CO2, but a Green E. coli.Frontiers in bioengineering and biotechnology · 2014Review
- Cyanobacteria as an Experimental Platform for Modifying Bacterial and Plant Photosynthesis.Frontiers in bioengineering and biotechnology · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe cyanobacterium Synechocystis sp. PCC 6803 is widely used for research on photosynthesis and circadian rhythms, and also finds application in sustainable biotechnologies. Synechocystis is naturally transformable and undergoes homologous recombination, which enables the development of a variety of tools for genetic and genomic manipulations. To generate multiple gene deletions and/or replacements, marker-less manipulation methods based on counter-selection are generally employed. Currently available methods require two transformation steps with different DNA plasmids.
resultsIn this study, we present a marker-less gene deletion and replacement strategy in Synechocystis sp. PCC 6803 which needs only a single transformation step. The method utilizes an nptI-sacB double selection cassette and exploits the ability of the cyanobacterium to undergo two successive genomic recombination events via double and single crossing-over upon application of appropriate selective procedures.
conclusionsBy reducing the number of cloning steps, this strategy will facilitate gene manipulation, gain-of-function studies, and automated screening of mutants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.