ArticleMalaria journal2014
Simple, sensitive and quantitative bioluminescence assay for determination of malaria pre-patent period.
Article in Malaria journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- Host cell traversal by Plasmodium parasites drives sterilizing T cell-mediated immunity following immunization.Nature microbiology · 2026Article
- Development of a Luciferase-Based In Vitro Assay to Evaluate the Efficacy of Anti-Cryptosporidial Drugs AgainstPharmaceuticals (Basel, Switzerland) · 2026Article
- The effect of dosage on the protective efficacy of whole-sporozoite formulations for immunization against malaria.NPJ vaccines · 2023Article
- SARS-CoV-2 decreases malaria severity in co-infected rodent models.Frontiers in cellular and infection microbiology · 2023Article
- Maria M. Mota: BringingFrontiers in cellular and infection microbiology · 2022Article
- Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection.PLoS neglected tropical diseases · 2021Article
- Trypanosoma brucei infection protects mice against malaria.PLoS pathogens · 2019Article
- γδ-T cells promote IFN-γ-dependentProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Updating the modified Thompson test by using whole-body bioluminescence imaging to replace traditional efficacy testing in experimental models of murine malaria.Malaria journal · 2019Article
- Dietary alterations modulate susceptibility to Plasmodium infection.Nature microbiology · 2017Article
- Bioluminescence Method forAntimicrobial agents and chemotherapy · 2017Article
- An ultrasensitive NanoLuc-based luminescence system for monitoring Plasmodium berghei throughout its life cycle.Malaria journal · 2016Article
- Efficient monitoring of the blood-stage infection in a malaria rodent model by the rotating-crystal magneto-optical method.Scientific reports · 2016Article
- Advances in molecular genetic systems in malaria.Nature reviews. Microbiology · 2015Review
- Towards genome-wide experimental genetics in the in vivo malaria model parasite Plasmodium berghei.Pathogens and global health · 2015Review
- Innate immunity induced by Plasmodium liver infection inhibits malaria reinfections.Infection and immunity · 2015Article
- Enlightening the malaria parasite life cycle: bioluminescent Plasmodium in fundamental and applied research.Frontiers in microbiology · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe first phase of malaria infection occurs in the liver and is clinically silent. Inside hepatocytes each Plasmodium sporozoite replicate into thousands of erythrocyte-infectious merozoites that when released into the blood stream result in clinical symptoms of the disease. The time between sporozoite inoculation and the appearance of parasites in the blood is defined as the pre-patent period, which is classically analysed by time-consuming and labor-intensive techniques, such as microscopy and PCR.
methodsLuciferase-expressing Plasmodium berghei parasites were used to measure pre-patent period of malaria infection in rodents using a bioluminescence assay that requires only one microliter of blood collected from the tail-vein. The accuracy and sensitivity of this new method was compared with conventional microscopy and PCR based techniques, and its capacity to measure the impact of anti-malarial interventions against the liver evaluated.
resultsThe described method is very sensitive allowing the detection of parasites during the first cycles of blood stage replication. It accurately translates differences in liver load due to inoculation of different sporozoite doses as well as a result of treatment with different primaquine regimens.
conclusionsA novel, simple, fast, and sensitive method to measure pre-patent period of malaria infection in rodents is described here. The sensitivity and accuracy of this new method is comparable to standard PCR and microscopy-based techniques, respectively.
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