ArticlePhysiological reports2013
Temperature and transmural region influence functional measurements in unloaded left ventricular cardiomyocytes.
Article in Physiological reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Heterogeneity of ventricular action potentials in neonatal rat cardiomyocytes and methodological aspects of patch clamp measurements.Frontiers in physiology · 2025Article
- Mechanical loading reveals an intrinsic cardiomyocyte stiffness contribution to diastolic dysfunction in murine cardiometabolic disease.The Journal of physiology · 2024Article
- Methods for assessing cardiac myofilament calcium sensitivity.Frontiers in physiology · 2023Review
- Contractile State Dependent Sarcomere Length Variability in Isolated Guinea-Pig Cardiomyocytes.Frontiers in physiology · 2022Article
- Membrane stabilizer Poloxamer 188 improves yield of primary isolated rat cardiomyocytes without impairing function.Physiological reports · 2020Article
- Large-Scale Contractility Measurements Reveal Large Atrioventricular and Subtle Interventricular Differences in Cultured Unloaded Rat Cardiomyocytes.Frontiers in physiology · 2020Article
- Voltage dependence of the CaMolecular and cellular biochemistry · 2018Article
- Transmural cellular heterogeneity in myocardial electromechanics.The journal of physiological sciences : JPS · 2018Article
- Myocardial relaxation is accelerated by fast stretch, not reduced afterload.Journal of molecular and cellular cardiology · 2017Article
- Cholesterol Depletion Alters Cardiomyocyte Subcellular Signaling and Increases Contractility.PloS one · 2016Article
- Myocyte contractility can be maintained by storing cells with the myosin ATPase inhibitor 2,3 butanedione monoxime.Physiological reports · 2015Article
- Exposure to phthalates affects calcium handling and intercellular connectivity of human stem cell-derived cardiomyocytes.PloS one · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Intact cardiomyocytes are increasingly being used to investigate the molecular mechanisms of contraction and to screen new therapeutic compounds. The function of the cardiomyocytes is often measured from the calcium transients and sarcomere length profiles. We studied the role of experimental temperature and transmural region on indices of function in freshly isolated, unloaded cardiomyocytes. Intact cardiomyocytes were isolated from the subendocardium, midmyocardium, and subepicardium of 3-month-old Sprague-Dawley rats. Myocytes from each region were studied at 25°C, 31°C, and 37°C. Cytosolic calcium transients were measured using Fura-2 fluorescence, whereas sarcomere length shortening and relengthening profiles were measured using high-speed video capture. For both the calcium transients and sarcomere length profiles, the time to peak and the time to half relaxation decreased significantly with increasing temperature. Increasing temperature also raised the minimum and maximum calcium levels of all cells. Of note, there was a reduced coefficient of variation (standard deviation divided by the mean) at higher temperatures for calcium fluorescence amplitudes, time to peak calcium, and rates of sarcomeric shortening and relengthening. The amplitudes and minimum of the calcium transients were significantly dependent on transmural region, and several sarcomere length parameters exhibited statistical interactions between temperature and transmural region. Together, these results show that biological variability can be reduced by performing experiments at 37°C rather than at room temperature, and by isolating cells from a specific transmural region. Adopting these procedures will improve the statistical power of subsequent analyses and increase the efficiency of future experiments.
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