ArticleFrontiers in plant science2013
Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmer-preferred cassava cultivars.
Article in Frontiers in plant science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 47 citations in OpenAlex.
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- Magnesium chelatase subunit D is not only required for chlorophyll biosynthesis and photosynthesis, but also affecting starch accumulation in Manihot esculenta Crantz.BMC plant biology · 2023Article
- CRISPR/Cas9: an advanced platform for root and tuber crops improvement.Frontiers in genome editing · 2023Review
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- Cassava mosaic disease and its management in Southeast Asia.Plant molecular biology · 2022Review
- Efficient Genetic Transformation and Regeneration of a Farmer-Preferred Cassava Cultivar From Ghana.Frontiers in plant science · 2021Article
- Article
- Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels.Methods and protocols · 2018Article
- Overexpression of rice thaumatin-like protein (Journal, genetic engineering & biotechnology · 2018Article
- New opportunities and challenges to engineer disease resistance in cassava, a staple food of African small-holder farmers.PLoS pathogens · 2017Review
- Gene expression atlas for the food security crop cassava.The New phytologist · 2017Article
- Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate.PloS one · 2017Article
- Sequencing wild and cultivated cassava and related species reveals extensive interspecific hybridization and genetic diversity.Nature biotechnology · 2016Article
- Increased bioavailable vitamin B6 in field-grown transgenic cassava for dietary sufficiency.Nature biotechnology · 2015Article
- Transition from somatic embryo to friable embryogenic callus in cassava: dynamic changes in cellular structure, physiological status, and gene expression profiles.Frontiers in plant science · 2015Article
- Factors influencing somatic embryogenesis, regeneration, and Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) cultivar TME14.Frontiers in plant science · 2015Article
- The potential of using biotechnology to improve cassava: a review.In vitro cellular & developmental biology. Plant : journal of the Tissue Culture AssociationReview
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6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cassava genetic transformation capacity is still mostly restricted to advanced laboratories in the USA, Europe and China; and its implementation and maintenance in African laboratories has remained scarce. The impact of transgenic technologies for genetic improvement of cassava will depend largely on the transfer of such capabilities to researchers in Africa, where cassava has an important socioeconomic niche. A major constraint to the development of genetic transformation technologies for cassava improvement has been the lack of an efficient and robust transformation and regeneration system. Despite the success achieved in genetic modification of few cassava cultivars, including the model cultivar 60444, transgenic cassava production remains difficult for farmer-preferred cultivars. In this study, a protocol for cultivar 60444 developed at ETH Zurich was successfully implemented and optimized to establish transformation of farmer-preferred cassava cultivars popular in east Africa. The conditions for production and proliferation of friable embryogenic calli (FEC) and Agrobacterium-mediated transformation were optimized for three east African farmer-preferred cultivars (Ebwanatereka, Kibandameno and Serere). Our results demonstrated transformation efficiencies of about 14-22 independent transgenic lines per 100 mg of FEC for farmer-preferred cultivars in comparison to 28 lines per 100 mg of the model cultivar 60444. The presence, integration and expression of the transgenes were confirmed by PCR, Southern blot analysis and histochemical GUS assay. This study reports the establishment of a cassava transformation platform at International Institute of Tropical Agriculture (IITA) hosted by Biosciences eastern and central Africa (BecA) hub in Kenya and provides the basis for transferring important traits such as virus resistance and prolonged shelf-life to farmer-preferred cultivars in east Africa. We anticipate that such platform will also be instrumental to transfer technologies to national agricultural research systems (NARS) in sub-Saharan Africa.
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