ArticleBreeding science2013
Efficient haploid and doubled haploid production from unfertilized ovule culture of gentians (Gentiana spp.).
Article in Breeding science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Autonomous development and regeneration of isolated rice egg cells in vitro in a fertilization-independent manner.Journal of experimental botany · 2026Article
- Identification of Candidate Genes Responsible for Flower Colour Intensity inFrontiers in plant science · 2022Article
- Overview of In Vitro and In Vivo Doubled Haploid Technologies.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Species with Haploid or Doubled Haploid Protocols.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Somatic Embryogenesis in the Family Gentianaceae and Its Biotechnological Application.Frontiers in plant science · 2019Review
- Development of basic technologies for improvement of breeding and cultivation of Japanese gentian.Breeding science · 2018Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Factors affecting reliable plant regeneration from unfertilized ovule culture of gentians (Gentiana spp.) were examined. Cold pretreatment (4°C) of flower buds enhanced or maintained production of embryo-like structure (ELS). When 43 genotypes were surveyed in two different labs, 40 of them produced ELSs ranging from 0.01 to 26.5 ELSs per flower bud. No ELSs could be obtained in three genotypes. A significant correlation (r = 0.64) was observed between the number of ELS per flower and the frequency of responding flower buds. Eight genotypes of G. triflora, which were used as common materials in two different labs, produced ELSs in both labs. The ploidy levels of a total of 1,515 regenerated plantlets were determined, revealing that the majority of these plants consisted of haploids (57.9%) and diploids (34.3%). However, the frequency of haploids and diploids was different between G. triflora and G. scabra, and G. triflora showed higher frequencies of haploids than G. scabra. When haploids were treated with oryzalin for chromosome doubling, diploids and tetraploids were obtained. These results demonstrate that the unfertilized ovule culture technique of gentians is a powerful tool for obtaining haploids and DHs because of its reproducible and reliable nature and application to a wide range of genotypes.
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Registered trials
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