ArticleNucleic acids research2014
The functions of DNA methylation by CcrM in Caulobacter crescentus: a global approach.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
85 citing papers in PubMed, 144 citations in OpenAlex.
- Genome replication and cell division control by the methylation-sensitive GcrA regulator in Agrobacterium tumefaciens.Nucleic acids research · 2026Article
- Type I restriction-modification systems regulate gene expression through DNA topology.Nucleic acids research · 2026Article
- Epigenetic Phase Variation in Bacterial Adaptation to Environmental Stress: A Narrative Review.Current microbiology · 2026Review
- Cytosine methylation contributes to the fitness ofiScience · 2026Article
- Epigenetic regulation in prokaryotes: transcriptional and phenotypic outcomes of DNA methyltransferase activity.FEMS microbiology reviews · 2026Review
- A comparison of short- and long-read whole-genome sequencing for microbial pathogen epidemiology.mSystems · 2025Article
- Long-read methylome analysis of Oleidesulfovibrio alaskensis G20 biofilm under copper stress.Scientific reports · 2025Article
- A comparison of short- and long-read whole genome sequencing for microbial pathogen epidemiology.bioRxiv : the preprint server for biology · 2025Article
- DNA methylation affects gene expression but not global chromatin structure inJournal of bacteriology · 2025Article
- Identifying DNA methylation types and methylated base positions from bacteria using nanopore sequencing with multi-scale neural network.Bioinformatics (Oxford, England) · 2025Article
- Article
- Ecological features of microbial community linked to stochastic and deterministic assembly processes in acid mine drainage.Applied and environmental microbiology · 2025Article
- Sequencing-based analysis of microbiomes.Nature reviews. Genetics · 2024Review
- DNA methylation by CcrM contributes to genome maintenance in the Agrobacterium tumefaciens plant pathogen.Nucleic acids research · 2024Article
- Comparison of CcrM-dependent methylation inJournal of bacteriology · 2024Article
- Review
- Moving toward the Inclusion of Epigenomics in Bacterial Genome Evolution: Perspectives and Challenges.International journal of molecular sciences · 2024Review
- Comparison of CcrM-dependent methylation inbioRxiv : the preprint server for biology · 2024Article
- AamA-mediated epigenetic control of genome-wide gene expression and phenotypic traits inMicrobial genomics · 2023Article
- High fidelity DNA strand-separation is the major specificity determinant in DNA methyltransferase CcrM's catalytic mechanism.Nucleic acids research · 2023Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
DNA methylation is involved in a diversity of processes in bacteria, including maintenance of genome integrity and regulation of gene expression. Here, using Caulobacter crescentus as a model, we exploit genome-wide experimental methods to uncover the functions of CcrM, a DNA methyltransferase conserved in most Alphaproteobacteria. Using single molecule sequencing, we provide evidence that most CcrM target motifs (GANTC) switch from a fully methylated to a hemi-methylated state when they are replicated, and back to a fully methylated state at the onset of cell division. We show that DNA methylation by CcrM is not required for the control of the initiation of chromosome replication or for DNA mismatch repair. By contrast, our transcriptome analysis shows that >10% of the genes are misexpressed in cells lacking or constitutively over-expressing CcrM. Strikingly, GANTC methylation is needed for the efficient transcription of dozens of genes that are essential for cell cycle progression, in particular for DNA metabolism and cell division. Many of them are controlled by promoters methylated by CcrM and co-regulated by other global cell cycle regulators, demonstrating an extensive cross talk between DNA methylation and the complex regulatory network that controls the cell cycle of C. crescentus and, presumably, of many other Alphaproteobacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.