ArticleProceedings of the National Academy of Sciences of the United States of America2014
Fatty acid synthesis is inhibited by inefficient utilization of unusual fatty acids for glycerolipid assembly.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 146 citations in OpenAlex.
- From wrinkled seeds to plant oil accumulation networks: the legacy of a Plant Physiology classic.Plant physiology · 2026Review
- Making fish oils in plants: from alpha to omega.The New phytologist · 2026Review
- Understanding the dynamic nature of plant lipid anabolic and catabolic metabolism is key to sustainable oilseed engineering.The New phytologist · 2026Review
- Triacylglycerol stability limits futile cycles and inhibition of carbon capture in oil-accumulating leaves.Plant physiology · 2025Article
- Born of frustration: the emergence of Camelina sativa as a platform for lipid biotechnology.Plant physiology · 2025Review
- Metabolic flux analysis to increase oil in seeds.Plant physiology · 2025Review
- Spatiotemporal transcriptome and metabolome landscapes of cotton somatic embryos.Nature communications · 2025Article
- REGULATOR OF FATTY ACID SYNTHESIS proteins regulate de novo fatty acid synthesis by modulating hetACCase distribution.The Plant cell · 2024Article
- Targeted engineering of camelina and pennycress seeds for ultrahigh accumulation of acetyl-TAG.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Unraveling the Impacts of Germination on the Volatile and Fatty Acid Profile of Intermediate Wheatgrass (Molecules (Basel, Switzerland) · 2024Article
- FatPlants: a comprehensive information system for lipid-related genes and metabolic pathways in plants.Database : the journal of biological databases and curation · 2024Article
- The Biosynthesis Pattern and Transcriptome Analysis ofPlants (Basel, Switzerland) · 2024Article
- Identification of triacylglycerol remodeling mechanism to synthesize unusual fatty acid containing oils.Nature communications · 2024Article
- Circadian clock factors regulate the first condensation reaction of fatty acid synthesis in Arabidopsis.Cell reports · 2023Article
- Functional Genome Analyses Reveal the Molecular Basis of Oil Accumulation in Developing Seeds of Castor Beans.International journal of molecular sciences · 2023Article
- Progress in understanding and improving oil content and quality in seeds.Frontiers in plant science · 2023Review
- Transcriptional regulation of oil biosynthesis in seed plants: Current understanding, applications, and perspectives.Plant communications · 2022Review
- Expression of Physaria longchain acyl-CoA synthetases and hydroxy fatty acid accumulation in transgenic Arabidopsis.Journal of plant physiology · 2022Article
- Enhanced production of hydroxy fatty acids in Arabidopsis seed through modification of multiple gene expression.Biotechnology for biofuels and bioproducts · 2022Article
- Can omic tools help generate alternative newer sources of edible seed oil?Plant direct · 2022Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Degradation of unusual fatty acids through β-oxidation within transgenic plants has long been hypothesized as a major factor limiting the production of industrially useful unusual fatty acids in seed oils. Arabidopsis seeds expressing the castor fatty acid hydroxylase accumulate hydroxylated fatty acids up to 17% of total fatty acids in seed triacylglycerols; however, total seed oil is also reduced up to 50%. Investigations into the cause of the reduced oil phenotype through in vivo [(14)C]acetate and [(3)H]2O metabolic labeling of developing seeds surprisingly revealed that the rate of de novo fatty acid synthesis within the transgenic seeds was approximately half that of control seeds. RNAseq analysis indicated no changes in expression of fatty acid synthesis genes in hydroxylase-expressing plants. However, differential [(14)C]acetate and [(14)C]malonate metabolic labeling of hydroxylase-expressing seeds indicated the in vivo acetyl-CoA carboxylase activity was reduced to approximately half that of control seeds. Therefore, the reduction of oil content in the transgenic seeds is consistent with reduced de novo fatty acid synthesis in the plastid rather than fatty acid degradation. Intriguingly, the coexpression of triacylglycerol synthesis isozymes from castor along with the fatty acid hydroxylase alleviated the reduced acetyl-CoA carboxylase activity, restored the rate of fatty acid synthesis, and the accumulation of seed oil was substantially recovered. Together these results suggest a previously unidentified mechanism that detects inefficient utilization of unusual fatty acids within the endoplasmic reticulum and activates an endogenous pathway for posttranslational reduction of fatty acid synthesis within the plastid.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.