ArticleGenome biology and evolution2014
Insights into the preservation of the homomorphic sex-determining chromosome of Aedes aegypti from the discovery of a male-biased gene tightly linked to the M-locus.
Article in Genome biology and evolution, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 50 citations in OpenAlex.
- Monoallelic knockout of r2d2 affects the antiviral RNAi response to Mayaro virus and the reproductive potential in Aedes aegypti.Parasites & vectors · 2026Article
- Insights on the sex determination, vector capacity and ecological biology from a chromosomal level genome of vector mosquito, Armigeres subulbatus.Infectious diseases of poverty · 2025Article
- Differential elimination of marked sex chromosomes enables production of nontransgenic male mosquitoes in a single strain.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Hybridization between Aedes aegypti and Aedes mascarensis mosquitoes leads to disruption of male sex determination.Communications biology · 2024Article
- The chromosome-scale genome assembly for the West Nile vector Culex quinquefasciatus uncovers patterns of genome evolution in mosquitoes.BMC biology · 2024Article
- A DBHS family member regulates male determination in the filariasis vector Armigeres subalbatus.Nature communications · 2023Article
- Marker-assisted mapping enables forward genetic analysis in Aedes aegypti, an arboviral vector with vast recombination deserts.Genetics · 2022Article
- Selective targeting of biting females to control mosquito-borne infectious diseases.Trends in parasitology · 2022Review
- Manipulating Insect Sex Determination Pathways for Genetic Pest Management: Opportunities and Challenges.Frontiers in bioengineering and biotechnology · 2022Review
- Article
- Irradiation induced inversions suppress recombination between the M locus and morphological markers in Aedes aegypti.BMC genetics · 2020Article
- CRISPR/Cas9 knockout of female-biased genes AeAct-4 or myo-fem in Ae. aegypti results in a flightless phenotype in female, but not male mosquitoes.PLoS neglected tropical diseases · 2020Article
- The genomes of a monogenic fly: views of primitive sex chromosomes.Scientific reports · 2020Article
- Improved reference genome of the arboviral vector Aedes albopictus.Genome biology · 2020Article
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- Article
- The Nix locus on the male-specific homologue of chromosome 1 in Aedes albopictus is a strong candidate for a male-determining factor.Parasites & vectors · 2018Article
- Functional analysis of the promoter of an early zygotic gene KLC2 in Aedes aegypti.Parasites & vectors · 2018Article
- Article
- The sequence of a male-specific genome region containing the sex determination switch in Aedes aegypti.Parasites & vectors · 2018Article
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10 authors at 1 institution in 1 country.
Funding
Abstract
The preservation of a homomorphic sex-determining chromosome in some organisms without transformation into a heteromorphic sex chromosome is a long-standing enigma in evolutionary biology. A dominant sex-determining locus (or M-locus) in an undifferentiated homomorphic chromosome confers the male phenotype in the yellow fever mosquito Aedes aegypti. Genetic evidence suggests that the M-locus is in a nonrecombining region. However, the molecular nature of the M-locus has not been characterized. Using a recently developed approach based on Illumina sequencing of male and female genomic DNA, we identified a novel gene, myo-sex, that is present almost exclusively in the male genome but can sporadically be found in the female genome due to recombination. For simplicity, we define sequences that are primarily found in the male genome as male-biased. Fluorescence in situ hybridization (FISH) on A. aegypti chromosomes demonstrated that the myo-sex probe localized to region 1q21, the established location of the M-locus. Myo-sex is a duplicated myosin heavy chain gene that is highly expressed in the pupa and adult male. Myo-sex shares 83% nucleotide identity and 97% amino acid identity with its closest autosomal paralog, consistent with ancient duplication followed by strong purifying selection. Compared with males, myo-sex is expressed at very low levels in the females that acquired it, indicating that myo-sex may be sexually antagonistic. This study establishes a framework to discover male-biased sequences within a homomorphic sex-determining chromosome and offers new insights into the evolutionary forces that have impeded the expansion of the nonrecombining M-locus in A. aegypti.
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