ArticleActa neuropathologica communications2014
Generation of iPSC lines from archived non-cryoprotected biobanked dura mater.
Article in Acta neuropathologica communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Postmortem Human Dura Mater Cells Exhibit Phenotypic, Transcriptomic and Genetic Abnormalities that Impact their Use for Disease Modeling.Stem cell reviews and reports · 2022Article
- Skin Mirrors Brain: A Chance for Alzheimer's Disease Research.Advances in experimental medicine and biology · 2021Article
- The application of in vitro-derived human neurons in neurodegenerative disease modeling.Journal of neuroscience research · 2021Review
- CRISPR-based functional genomics for neurological disease.Nature reviews. Neurology · 2020Review
- Exploratory Analysis of iPSCS-Derived Neuronal Cells as Predictors of Diagnosis and Treatment of Alzheimer Disease.Brain sciences · 2020Article
- Ultrastructural morphology is distinct among primary progenitor cell isolates from normal, inflamed, and cryopreserved equine hoof tissue and CD105In vitro cellular & developmental biology. Animal · 2019Article
- Important advances in Alzheimer's disease from the use of induced pluripotent stem cells.Journal of biomedical science · 2019Review
- Leptomeninges-Derived Induced Pluripotent Stem Cells and Directly Converted Neurons From Autopsy Cases With Varying Neuropathologic Backgrounds.Journal of neuropathology and experimental neurology · 2018Article
- Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors.Journal of visualized experiments : JoVE · 2017Article
- Needs and Requirements of Modern Biobanks on the Example of Dystonia Syndromes.Frontiers in neurology · 2017Article
- Alzheimer disease research in the 21st century: past and current failures, new perspectives and funding priorities.Oncotarget · 2016Article
- Accelerating stem cell trials for Alzheimer's disease.The Lancet. Neurology · 2016Review
- Efficient Generation of Induced Pluripotent Stem and Neural Progenitor Cells From Acutely Harvested Dura Mater Obtained During Ventriculoperitoneal Shunt Surgery.World neurosurgery · 2015Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
backgroundInduced pluripotent stem cells (iPSCs) derived from patients with neurodegenerative disease generally lack neuropathological confirmation, the gold standard for disease classification and grading of severity. The use of tissue with a definitive neuropathological diagnosis would be an ideal source for iPSCs. The challenge to this approach is that the majority of biobanked brain tissue was not meant for growing live cells, and thus was not frozen in the presence of cryoprotectants such as DMSO.
resultsWe report the generation of iPSCs from frozen non-cryoprotected dural tissue stored at -80°C for up to 11 years. This autopsy cohort included subjects with Alzheimer's disease and four other neurodegenerative diseases.
conclusionsDisease-specific iPSCs can be generated from readily available, archival biobanked tissue. This allows for rapid expansion of generating iPSCs with confirmed pathology as well as allowing access to rare patient variants that have been banked.
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