ArticleInvestigative ophthalmology & visual science2014
Evaluation of retinal nerve fiber layer thickness and axonal transport 1 and 2 weeks after 8 hours of acute intraocular pressure elevation in rats.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 67 citations in OpenAlex.
- Increase of retinal layer thickness in a rat model of acute intraocular hypertension.Experimental eye research · 2026Article
- A system for producing controlled elevation of intraocular pressure in awake Brown Norway rats.Experimental eye research · 2025Article
- Review: Neuroprotective Nanocarriers in Glaucoma.Pharmaceuticals (Basel, Switzerland) · 2024Review
- Impact of Acute Ocular Hypertension on Retinal Ganglion Cell Loss in Mice.Translational vision science & technology · 2024Article
- Topical Administration of a Nanoformulation of Chitosan-Hyaluronic Acid-Epoetin Beta in a Rat Model of Glaucoma.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Retrograde Axonal Transport of Liposomes from Peripheral Tissue to Spinal Cord and DRGs by Optimized Phospholipid and CTB Modification.International journal of molecular sciences · 2022Article
- The Role of Axonal Transport in Glaucoma.International journal of molecular sciences · 2022Review
- Mechanisms of retinal ganglion cell injury following acute increases in intraocular pressure.Frontiers in ophthalmology · 2022Review
- Retinal Ganglion Cell Degeneration in a Rat Magnetic Bead Model of Ocular Hypertensive Glaucoma.Translational vision science & technology · 2021Article
- Association of Optical Coherence Tomography With Longitudinal Neurodegeneration in Veterans With Chronic Mild Traumatic Brain Injury.JAMA network open · 2020Article
- Monitoring retinal responses to acute intraocular pressure elevation in rats with visible light optical coherence tomography.Neurophotonics · 2019Article
- Relationship between preoperative high intraocular pressure and retinal nerve fibre layer thinning after glaucoma surgery.Scientific reports · 2019Observational
- Temporal change of retinal nerve fiber layer reflectance speckle in normal and hypertensive retinas.Experimental eye research · 2019Article
- Early-Stage Ocular Hypertension Alters Retinal Ganglion Cell Synaptic Transmission in the Visual Thalamus.Frontiers in cellular neuroscience · 2019Article
- Association of Retinal Neurodegeneration on Optical Coherence Tomography With Dementia: A Population-Based Study.JAMA neurology · 2018Article
- Assessing Trends in Functional and Structural Characteristics: A Survey of Statistical Methods With an Example From Ophthalmology.Translational vision science & technology · 2018Article
- Article
- Ocular fundus pulsations within the posterior rat eye: Chorioscleral motion and response to elevated intraocular pressure.Scientific reports · 2017Article
- The P2X7 receptor links mechanical strain to cytokine IL-6 up-regulation and release in neurons and astrocytes.Journal of neurochemistry · 2017Article
- MicroRNA regulation in an animal model of acute ocular hypertension.Acta ophthalmologica · 2017Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo compare in vivo retinal nerve fiber layer thickness (RNFLT) and axonal transport at 1 and 2 weeks after an 8-hour acute IOP elevation in rats.
methodsForty-seven adult male Brown Norway rats were used. Procedures were performed under anesthesia. The IOP was manometrically elevated to 50 mm Hg or held at 15 mm Hg (sham) for 8 hours unilaterally. The RNFLT was measured by spectral-domain optical coherence tomography. Anterograde and retrograde axonal transport was assessed from confocal scanning laser ophthalmoscopy imaging 24 hours after bilateral injections of 2 μL 1% cholera toxin B-subunit conjugated to AlexaFluor 488 into the vitreous or superior colliculi, respectively. Retinal ganglion cell (RGC) and microglial densities were determined using antibodies against Brn3a and Iba-1.
resultsThe RNFLT in experimental eyes increased from baseline by 11% at 1 day (P < 0.001), peaked at 19% at 1 week (P < 0.0001), remained 11% thicker at 2 weeks (P < 0.001), recovered at 3 weeks (P > 0.05), and showed no sign of thinning at 6 weeks (P > 0.05). There was no disruption of anterograde transport at 1 week (superior colliculi fluorescence intensity, 75.3 ± 7.9 arbitrary units [AU] for the experimental eyes and 77.1 ± 6.7 AU for the control eyes) (P = 0.438) or 2 weeks (P = 0.188). There was no obstruction of retrograde transport at 1 week (RCG density, 1651 ± 153 per mm(2) for the experimental eyes and 1615 ± 135 per mm(2) for the control eyes) (P = 0.63) or 2 weeks (P = 0.25). There was no loss of Brn3a-positive RGC density at 6 weeks (P = 0.74) and no increase in microglial density (P = 0.92).
conclusionsAcute IOP elevation to 50 mm Hg for 8 hours does not cause a persisting axonal transport deficit at 1 or 2 weeks or a detectable RNFLT or RGC loss by 6 weeks but does lead to transient RNFL thickening that resolves by 3 weeks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.