Evidence map›Paper›PMID 24397987›Full record

ArticleBiomaterials2014

Gene silencing in human aortic smooth muscle cells induced by PEI-siRNA complexes released from dip-coated electrospun poly(ethylene terephthalate) grafts.

Christoph S Nabzdyk, Maggie C Chun, Hunter S Oliver-Allen, Saif G Pathan, Matthew D Phaneuf, Jin-Oh You, Leena K Pradhan-Nabzdyk, Frank W LoGerfo

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.6field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Gene Silencing via PDA/ERK2-siRNA-Mediated Electrospun Fibers for Peritendinous Antiadhesion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019
    Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Article
  11. Technologies for controlled, local delivery of siRNA.Journal of controlled release : official journal of the Controlled Release Society · 2015
    Review
  12. Review
  13. Review
  14. Review
  15. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Christoph S NabzdykDivision of Vascular and Endovascular Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; Department of Surgery, Tufts Medical Center, Tufts University School of Medicine, Boston, MA, USA.
Maggie C ChunDivision of Vascular and Endovascular Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Hunter S Oliver-AllenDivision of Vascular and Endovascular Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Saif G PathanBioSurfaces, Inc., Ashland, MA, USA.
Matthew D PhaneufBioSurfaces, Inc., Ashland, MA, USA.
Jin-Oh YouHarvard School of Engineering and Applied Sciences, Cambridge, MA, USA; Department of Engineering Chemistry, Chungbuk National University, Cheongju, Republic of Korea.
Leena K Pradhan-NabzdykDivision of Vascular and Endovascular Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA. Electronic address: lpradhan@bidmc.harvard.edu.
Frank W LoGerfoDivision of Vascular and Endovascular Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA. Electronic address: flogerfo@bidmc.harvard.edu.
Harvard University · USAshland (United States) · USChungbuk National University · KRTufts Medical Center · US

Funding

HARVARD-LONGWOOD RESEARCH TRAINING IN VASCULAR SURGERYT32HL007734 · NHLBI · BETH ISRAEL DEACONESS MEDICAL CENTER · PI FERRAN, CHRISTIANE, LOGERFO, FRANK W · 1993 to 2023
$10.5M
MECHANISMS OF PROSTHETIC ARTERIAL GRAFT FAILURER01HL021796 · NHLBI · NEW ENGLAND DEACONESS HOSPITAL · PI FERRAN, CHRISTIANE, LOGERFO, FRANK W · 1987 to 2020
$9.0M
Genetic Engineering of Vein Bypass Grafts in Vascular and Cardiovascular SurgeryR01HL086741 · NHLBI · BETH ISRAEL DEACONESS MEDICAL CENTER · PI BHASIN, MANOJ, FERRAN, CHRISTIANE · 2007 to 2022
$7.4M
NHLBI NIH HHS 2R01 HL086741NHLBI NIH HHS 5R01 HL021796NHLBI NIH HHS R01 HL021796NHLBI NIH HHS R01 HL086741NHLBI NIH HHS T32 HL007734
6 · The paper itself

Abstract

An excessive tissue response to prosthetic arterial graft material leads to intimal hyperplasia (IH), the leading cause of late graft failure. Seroma and abnormal capsule formation may also occur after prosthetic material implantation. The matricellular protein Thrombospondin-2 (TSP-2) has shown to be upregulated in response to biomaterial implantation. This study evaluates the uptake and release of small interfering RNA (siRNA) from unmodified and surface functionalized electrospun PET graft materials. ePET graft materials were synthesized using electrospinning technology. Subsets of the ePET materials were then chemically modified to create surface functional groups. Unmodified and surface-modified ePET grafts were dip-coated in siRNAs alone or siRNAs complexed with transfection reagents polyethyleneimine (PEI) or Lipofectamine RNAiMax. Further, control and TSP-2 siRNA-PEI complex treated ePET samples were placed onto a confluent layer of human aortic smooth muscle cells (AoSMCs). Complexation of all siRNAs with PEI led to a significant increase in adsorption to unmodified ePET. TSP-2 siRNA-PEI released from unmodified-ePET silenced TSP-2 in AoSMC. Regardless of the siRNA-PEI complex evaluated, AoSMC migrated into the ePET. siRNA-PEI complexes delivered to AoSMC from dip-coated ePET can result in gene knockdown. This methodology for siRNA delivery may improve the tissue response to vascular and other prosthetics.

Indexed as

AdsorptionAortaCell AdhesionCell ShapeCoated Materials, BiocompatibleGene Knockdown TechniquesGene SilencingHumansMyocytes, Smooth MusclePolyethylene GlycolsPolyethyleneiminePolyethylene TerephthalatesReproducibility of ResultsRNA, MessengerRNA, Small InterferingTransfectionCoated Materials, BiocompatiblePolyethylene GlycolsPolyethyleneiminePolyethylene TerephthalatesRNA, MessengerRNA, Small InterferingHuman aortic smooth muscle cellsPolyesterSilencing RNAThrombospondin-2Transfection reagents

Identifiers

PMID24397987
PMCPMC3929209
OpenAlexW2007809075

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.