ArticleAnalytical chemistry2014
Quantitation of cellular metabolic fluxes of methionine.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 41 citations in OpenAlex.
- Role of methionine metabolism in cancer: recent advances in molecular mechanisms and therapeutic implications.Experimental hematology & oncology · 2026Review
- Revealing the spatiotemporal dynamics of methionine metabolism with a genetically encoded single-fluorophore biosensor.bioRxiv : the preprint server for biology · 2026Article
- Methionine restriction improves lipid metabolism disorders and enhances immunity in obese mice through liver lipid remodeling.Frontiers in nutrition · 2026Article
- A new gut pathogenic bacteria and its metabolites promote colorectal cancer development and act as non-invasive early diagnostic biomarkers.Gut microbes · 2025Article
- The role of BCancer & metabolism · 2025Article
- Article
- Vitamin BScience advances · 2023Article
- Biosynthetic Profile in the Co-culture of Aspergillus sydowii and Bacillus subtilis to Produce Novel Benzoic Derivatives.Microbial ecology · 2023Article
- Homozygous MTAP deletion in primary human glioblastoma is not associated with elevation of methylthioadenosine.Nature communications · 2021Article
- Serine, glycine and one‑carbon metabolism in cancer (Review).International journal of oncology · 2021Review
- Global metabolomics profiling of colorectal cancer in Malaysian patients.BioImpacts : BI · 2021Article
- Polyamine pathway activity promotes cysteine essentiality in cancer cells.Nature metabolism · 2020Article
- Inhibition of DNMT1 and ERRα crosstalk suppresses breast cancer via derepression of IRF4.Oncogene · 2020Article
- One-carbon metabolism, folate, zinc and translation.Microbial biotechnology · 2020Article
- Formate metabolism in health and disease.Molecular metabolism · 2020Review
- Methionine metabolism and methyltransferases in the regulation of aging and lifespan extension across species.Aging cell · 2019Review
- Studying metabolic flux adaptations in cancer through integrated experimental-computational approaches.BMC biology · 2019Review
- ElemCor: accurate data analysis and enrichment calculation for high-resolution LC-MS stable isotope labeling experiments.BMC bioinformatics · 2019Article
- Canonical Wnt is inhibited by targeting one-carbon metabolism through methotrexate or methionine deprivation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Perinatal high fat diet and early life methyl donor supplementation alter one carbon metabolism and DNA methylation in the brain.Journal of neurochemistry · 2018Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Methionine is an essential proteogenic amino acid. In addition, it is a methyl donor for DNA and protein methylation and a propylamine donor for polyamine biosynthesis. Both the methyl and propylamine donation pathways involve metabolic cycles, and methods are needed to quantitate these cycles. Here, we describe an analytical approach for quantifying methionine metabolic fluxes that accounts for the mixing of intracellular and extracellular methionine pools. We observe that such mixing prevents isotope tracing experiments from reaching the steady state due to the large size of the media pools and hence precludes the use of standard stationary metabolic flux analysis. Our approach is based on feeding cells with (13)C methionine and measuring the isotope-labeling kinetics of both intracellular and extracellular methionine by liquid chromatography-mass spectrometry (LC-MS). We apply this method to quantify methionine metabolism in a human fibrosarcoma cell line and study how methionine salvage pathway enzyme methylthioadenosine phosphorylase (MTAP), frequently deleted in cancer, affects methionine metabolism. We find that both transmethylation and propylamine transfer fluxes amount to roughly 15% of the net methionine uptake, with no major changes due to MTAP deletion. Our method further enables the quantification of flux through the pro-tumorigenic enzyme ornithine decarboxylase, and this flux increases 2-fold following MTAP deletion. The analytical approach used to quantify methionine metabolic fluxes is applicable for other metabolic systems affected by mixing of intracellular and extracellular metabolite pools.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.