ArticleMolecular microbiology2014
EmrA1 membrane fusion protein of Francisella tularensis LVS is required for resistance to oxidative stress, intramacrophage survival and virulence in mice.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 29 citations in OpenAlex.
- The impact of EmrA deficiency on antibiotic-susceptibility and virulence- and stone-associated phenotypes inFrontiers in microbiology · 2026Article
- Role of thioredoxin reductase (TrxB) in oxidative stress response ofJournal of bacteriology · 2025Article
- TolC and EmrA1 contribute toJournal of bacteriology · 2024Article
- Stress responses in an Arctic microalga (Pelagophyceae) following sudden salinity change revealed by gene expression analysis.Communications biology · 2024Article
- Implication of the σMicrobiology spectrum · 2023Article
- Tularemia treatment: experimental and clinical data.Frontiers in microbiology · 2023Review
- ThioredoxinA1 Controls the Oxidative Stress Response of Francisella tularensis Live Vaccine Strain (LVS).Journal of bacteriology · 2022Article
- An AraC/XylS Family Transcriptional Regulator Modulates the Oxidative Stress Response of Francisella tularensis.Journal of bacteriology · 2021Article
- Aim2 and Nlrp3 Are Dispensable for Vaccine-Induced Immunity against Francisella tularensis Live Vaccine Strain.Infection and immunity · 2021Article
- Structural and functional analysis of the Francisella lysine decarboxylase as a key actor in oxidative stress resistance.Scientific reports · 2021Article
- Genetic Determinants of Antibiotic Resistance inFrontiers in microbiology · 2021Review
- Contributions of TolC Orthologs toInfection and immunity · 2019Article
- Stringent response governs the oxidative stress resistance and virulence of Francisella tularensis.PloS one · 2019Article
- Role of peroxiredoxin of the AhpC/TSA family in antioxidant defense mechanisms of Francisella tularensis.PloS one · 2019Article
- Characterization of a Unique Outer Membrane Protein Required for Oxidative Stress Resistance and Virulence of Francisella tularensis.Journal of bacteriology · 2018Article
- Live Attenuated Tularemia Vaccines for Protection Against Respiratory Challenge With VirulentFrontiers in cellular and infection microbiology · 2018Review
- The Ability to Acquire Iron Is Inversely Related to Virulence and the Protective Efficacy ofFrontiers in microbiology · 2018Article
- Components of the type six secretion system are substrates of Francisella tularensis Schu S4 DsbA-like FipB protein.Virulence · 2016Article
- Elucidation of a mechanism of oxidative stress regulation in Francisella tularensis live vaccine strain.Molecular microbiology · 2016Article
- Antioxidant Defenses of Francisella tularensis Modulate Macrophage Function and Production of Proinflammatory Cytokines.The Journal of biological chemistry · 2016Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Francisella tularensis is a category A biodefence agent that causes a fatal human disease known as tularaemia. The pathogenicity of F. tularensis depends on its ability to persist inside host immune cells primarily by resisting an attack from host-generated reactive oxygen and nitrogen species (ROS/RNS). Based on the ability of F. tularensis to resist high ROS/RNS levels, we have hypothesized that additional unknown factors act in conjunction with known antioxidant defences to render ROS resistance. By screening a transposon insertion library of F. tularensis LVS in the presence of hydrogen peroxide, we have identified an oxidant-sensitive mutant in putative EmrA1 (FTL_0687) secretion protein. The results demonstrate that the emrA1 mutant is highly sensitive to oxidants and several antimicrobial agents, and exhibits diminished intramacrophage growth that can be restored to wild-type F. tularensis LVS levels by either transcomplementation, inhibition of ROS generation or infection in NADPH oxidase deficient (gp91Phox(-/-)) macrophages. The emrA1 mutant is attenuated for virulence, which is restored by infection in gp91Phox(-/-) mice. Further, EmrA1 contributes to oxidative stress resistance by affecting secretion of Francisella antioxidant enzymes SodB and KatG. This study exposes unique links between transporter activity and the antioxidant defence mechanisms of F. tularensis.
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