ArticleMicrobial cell factories2014
Metabolic engineering of Pseudomonas sp. strain VLB120 as platform biocatalyst for the production of isobutyric acid and other secondary metabolites.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 83 citations in OpenAlex.
- Integrating synthetic biology and process engineering for enhanced isobutanol biosynthesis yield and sustainability.Biotechnology for biofuels and bioproducts · 2026Review
- Model-Guided Rational Construction of Escherichia coli Synthetic Consortia for Enhanced 2-Methylbutyric Acid Production.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Microbial host engineering for sustainable isobutanol production from renewable resources.Applied microbiology and biotechnology · 2024Review
- Maximizing Heterologous Expression of Engineered Type I Polyketide Synthases: Investigating Codon Optimization Strategies.ACS synthetic biology · 2023Article
- Role of Polysaccharides from Marine Seaweed as Feed Additives for Methane Mitigation in Ruminants: A Critical Review.Polymers · 2023Review
- Co-feeding enhances the yield of methyl ketones.Journal of industrial microbiology & biotechnology · 2023Article
- Metabolic engineering ofMetabolic engineering communications · 2022Article
- Transporter-Driven Engineering of a Genetic Biosensor for the Detection and Production of Short-Branched Chain Fatty Acids inFrontiers in bioengineering and biotechnology · 2022Article
- Micro-aerobic production of isobutanol with engineeredEngineering in life sciences · 2021Article
- EngineeringEngineering in life sciences · 2020Article
- A Review of the Biotechnological Production of Methacrylic Acid.Frontiers in bioengineering and biotechnology · 2020Review
- Bioengineering of anaerobic digestion for volatile fatty acids, hydrogen or methane production: A critical review.Bioengineered · 2019Review
- Article
- The crystal structure of D-xylonate dehydratase reveals functional features of enzymes from the Ilv/ED dehydratase family.Scientific reports · 2018Article
- Engineering alternative isobutanol production platforms.AMB Express · 2015Article
- 2-Keto acids based biosynthesis pathways for renewable fuels and chemicals.Journal of industrial microbiology & biotechnology · 2015Review
- Engineering mediator-based electroactivity in the obligate aerobic bacterium Pseudomonas putida KT2440.Frontiers in microbiology · 2015Article
- A microbial platform for renewable propane synthesis based on a fermentative butanol pathway.Biotechnology for biofuels · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOver the recent years the production of Ehrlich pathway derived chemicals was shown in a variety of hosts such as Escherichia coli, Corynebacterium glutamicum, and yeast. Exemplarily the production of isobutyric acid was demonstrated in Escherichia coli with remarkable titers and yields. However, these examples suffer from byproduct formation due to the fermentative growth mode of the respective organism. We aim at establishing a new aerobic, chassis for the synthesis of isobutyric acid and other interesting metabolites using Pseudomonas sp. strain VLB120, an obligate aerobe organism, as host strain.
resultsThe overexpression of kivd, coding for a 2-ketoacid decarboxylase from Lactococcus lactis in Ps. sp. strain VLB120 enabled for the production of isobutyric acid and isobutanol via the valine synthesis route (Ehrlich pathway). This indicates the existence of chromosomally encoded alcohol and aldehyde dehydrogenases catalyzing the reduction and oxidation of isobutyraldehyde. In addition we showed that the strain possesses a complete pathway for isobutyric acid metabolization, channeling the compound via isobutyryl-CoA into valine degradation. Three key issues were addressed to allow and optimize isobutyric acid synthesis: i) minimizing isobutyric acid degradation by host intrinsic enzymes, ii) construction of suitable expression systems and iii) streamlining of central carbon metabolism finally leading to production of up to 26.8 ± 1.5 mM isobutyric acid with a carbon yield of 0.12 ± 0.01 g g(glc)⁻¹.
conclusionThe combination of an increased flux towards isobutyric acid using a tailor-made expression system and the prevention of precursor and product degradation allowed efficient production of isobutyric acid in Ps. sp. strain VLB120. This will be the basis for the development of a continuous reaction process for this bulk chemicals.
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