Evidence map›Paper›PMID 24397211›Full record

ArticleGene expression2013

Determination of relative Notch1 and gamma-secretase-related gene expression in puromycin-treated microdissected rat kidneys.

Damir Simic, Frank Simutis, Catherine Euler, Christina Thurby, W Mike Peden, R Todd Bunch, Gary Pilcher, Thomas Sanderson, Terry Van Vleet

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gene expression, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Damir SimicBristol-Myers Squibb Co., Drug Safety Evaluation, Mt. Vernon, IN, 47620, USA. damir.simic@bms.com
Frank SimutisBristol-Myers Squibb Co., Drug Safety Evaluation, Mt. Vernon, IN, 47620, USA.
Catherine EulerBristol-Myers Squibb Co., Drug Safety Evaluation, Mt. Vernon, IN, 47620, USA.
Christina ThurbyBristol-Myers Squibb Co., Drug Safety Evaluation, Mt. Vernon, IN, 47620, USA.
W Mike PedenBristol-Myers Squibb Co., Drug Safety Evaluation, Mt. Vernon, IN, 47620, USA.
R Todd BunchBristol-Myers Squibb Co., Drug Safety Evaluation, Mt. Vernon, IN, 47620, USA.
Gary PilcherBristol-Myers Squibb Co., Drug Safety Evaluation, Mt. Vernon, IN, 47620, USA.
Thomas SandersonBristol-Myers Squibb Co., Drug Safety Evaluation, Mt. Vernon, IN, 47620, USA.
Terry Van VleetBristol-Myers Squibb Co., Drug Safety Evaluation, Mt. Vernon, IN, 47620, USA.
Bristol-Myers Squibb (Germany) · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Notch signaling pathways are involved in the regulation of cell differentiation and are highly conserved across species. Notch ligand binding leads to gamma-secretase-mediated proteolytic cleavage of the Notch receptor releasing the Notch intracellular domain, resulting in its subsequent translocation into the nucleus and gene expression regulation. To investigate the level of expression of Notch signaling pathway components in microanatomic regions following renal injury, kidneys from untreated, vehicle control, and puromycin aminonucleoside (PA, 150 mg/kg)-treated rats were evaluated. Frozen tissue sections from rats were microdissected using laser capture microdissection (LCM) to obtain glomeruli, cortical (proximal) tubules, and collecting ducts, and relative gene expression levels of Presenilin1, Notch1 and Hes1 were determined. In untreated rats, the Notch1 expression in glomeruli was higher than in the proximal tubules and similar to that in collecting ducts, whereas Presenilin1 and Hes1 expressions were highest in the collecting ducts, followed by cortical tubules and glomeruli. Following PA-induced renal injury, Hes1 gene expression increased significantly in the glomeruli and tubules compared to the collecting ducts where no injury was observed microscopically. Although these data present some evidence of change in Notch signaling related to injury, the expression of Presenilin1, Notch1, and Hes1 in the microanatomic regions of the kidney following PA treatment were not significantly different when compared to controls. These results demonstrate that there are differences in Notch-related gene expression in the different microanatomic regions of the kidneys in rats and suggest a minimal role for Notch in renal injury induced by PA. In addition, this work shows that LCM coupled with the RT-PCR can be used to determine the relative differences in target gene expression within regions of a complex organ.

Indexed as

Amyloid Precursor Protein SecretasesAnimalsBasic Helix-Loop-Helix ProteinsFemaleGene ExpressionHomeodomain ProteinsKidneyPresenilin-1PuromycinRatsRats, Sprague-DawleyReceptor, Notch1Transcription Factor HES-1Amyloid Precursor Protein SecretasesBasic Helix-Loop-Helix ProteinsHes1 protein, ratHomeodomain ProteinsNotch1 protein, ratPresenilin-1PuromycinReceptor, Notch1Transcription Factor HES-1

Identifiers

PMID24397211
PMCPMC8750201
OpenAlexW2094281098

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.