ArticleGene expression2013
MicroRNAs synergistically regulate milk fat synthesis in mammary gland epithelial cells of dairy goats.
Article in Gene expression, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 65 citations in OpenAlex.
- Differential Expression of MicroRNAs in the Mammary Gland of Lactating and Dry Murciano-Granadina Goats.Animals : an open access journal from MDPI · 2026Article
- Milk Lipids as Bioactive Modulators of the Bacterial Proteome: Mechanisms Linking Dairy Management to Microbial Performance.Animals : an open access journal from MDPI · 2026Review
- Article
- Review
- Analysis of miRNAs in milk of four livestock species.BMC genomics · 2024Article
- Comparison of miR-106b, miR-191, and miR-30d expression dynamics in milk with regard to its composition in Holstein and Ayrshire cows.Animal bioscience · 2024Article
- Periconceptional and Prenatal Exposure to Metals and Extracellular Vesicle and Particle miRNAs in Human Milk: A Pilot Study.Exposure and health · 2023Article
- Seasonal Changes in the Antioxidant Activity and Biochemical Parameters of Goat Milk.Animals : an open access journal from MDPI · 2023Article
- Regulation of adipogenesis by exosomal milk miRNA.Reviews in endocrine & metabolic disorders · 2023Review
- circITGB1 Regulates Adipocyte Proliferation and Differentiation via the miR-23a/ARRB1 Pathway.International journal of molecular sciences · 2023Article
- Article
- An Integrated Analysis of Lactation-Related miRNA and mRNA Expression Profiles in Donkey Mammary Glands.Genes · 2022Article
- Bta-miR-33a affects gene expression and lipid levels in Chinese Holstein mammary epithelial cells.Archives animal breeding · 2022Article
- The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep.Non-coding RNA · 2021Review
- Comparative analysis of dietary exosome-derived microRNAs from human, bovine and caprine colostrum and mature milk.Journal of animal science and technology · 2021Article
- miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies.International journal of molecular sciences · 2021Review
- Review
- cAMP Response Element Binding Protein 1 (CREB1) Promotes Monounsaturated Fatty Acid Synthesis and Triacylglycerol Accumulation in Goat Mammary Epithelial Cells.Animals : an open access journal from MDPI · 2020Article
- Influence of Pasture on Stearoyl-CoA Desaturase and miRNA 103 Expression in Goat Milk: Preliminary Results.Animals : an open access journal from MDPI · 2019Article
- Comparative transcriptome analysis to investigate the potential role of miRNAs in milk protein/fat quality.Scientific reports · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synergistic regulation among microRNAs (miRNAs) is important to understand the mechanisms underlying the complex molecular regulatory networks in goats. Goat milk fat synthesis is driven by a gene network that involves many biological processes in the mammary gland. These biological processes are affected by several miRNAs rather than a single miRNA. Therefore, identifying synergistic miRNAs is necessary to further understand the functions of miRNAs and the metabolism of goat milk fat synthesis. Using qRT-PCR, we assessed the expression of 11 miRNAs that have the potential to regulate milk fat synthesis in the goat mammary gland. Six of these miRNAs exhibited expression during the lactation cycle. Additionally, we also found that prolactin, the key hormone that regulates lactation, promotes the expression of four miRNAs (miR-23a, miR-27b, miR-103, and miR-200a). Further functional analysis showed that overexpression of all four miRNAs by using recombinant adenovirus in goat mammary gland epithelial cells can affect gene mRNA expression associated with milk fat synthesis. Specifically, elevated miR-200a expression suppressed the mRNA expression of genes involved in fat droplet formation. To analyze the synergistic regulation among these four miRNAs (miR-23a, miR-27b, miR-103, and miR-200a), we used the Pearson correlation coefficient to evaluate the correlation between their expression levels in 30 lactating goats. As a result, we found a strong correlation and mutual regulation between three miRNA pairs (miR-23a and miR-27b, miR-103 and miR-200a, miR-27b and miR-200a). This study provides the first experimental evidence that miRNA expression is synergistically regulated in the goat mammary gland and has identified the potential biological role of miRNAs in goat milk fat synthesis. The identification of synergistic miRNAs is a crucial step for further understanding the molecular network of milk fat synthesis at a system-wide level.
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