Evidence map›Paper›PMID 24396731›Full record

ArticleCurrent chemical genomics and translational medicine2013

A Biochemical/Biophysical Assay Dyad for HTS-Compatible Triaging of Inhibitors of the HIV-1 Nef/Hck SH3 Interaction.

Sebastian Breuer, Sheryll Espinola, Xavier Morelli, Bruce E Torbett, Stefan T Arold, Ingo H Engels

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current chemical genomics and translational medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sebastian BreuerDepartment of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Sheryll EspinolaGenomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Dr, San Diego, CA 92121, USA.
Xavier MorelliCRCM, CNRS UMR7258, Laboratory of Integrative Structural and Chemical Biology (ISCB); INSERM, U1068; Institut Paoli-Calmettes; Aix-Marseille Université, UM105, F-13009, Marseille, France.
Bruce E TorbettDepartment of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Stefan T AroldDepartment of Biochemistry and Molecular Biology, Unit 1000, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA ; King Abdullah University of Science and Technology (KAUST), Division of Biological and Environmental Sciencesand Engineering, Computational Bioscience Research Center, Thuwal 23955-6900, Saudi Arabia.
Ingo H EngelsGenomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Dr, San Diego, CA 92121, USA.

Funding

STRUCTURE-BASED MECHANISMS FOR RESISTANCE TO PROTEASE INHIBITORSP01GM083658 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI OLSON, ARTHUR J. · 2008 to 2012
$9.6M
Anti-HIV Gene Delivery to Human Hematopoietic CellsR01HL091219 · NHLBI · SCRIPPS RESEARCH INSTITUTE, THE · PI TORBETT, BRUCE EDWARD · 2007 to 2010
$1.6M
NHLBI NIH HHS R01 HL091219NIGMS NIH HHS P01 GM083658
6 · The paper itself

Abstract

The current treatment regimens for HIV include over 20 anti-retrovirals. However, adverse drug effects and the emergence of drug resistance necessitates the continued improvement of the existing drug classes as well as the development of novel drugs that target as yet therapeutically unexploited viral and cellular pathways. Here we demonstrate a strategy for the discovery of protein-protein interaction inhibitors of the viral pathogenicity factor HIV-1 Nef and its interaction with the host factor SH3. A combination of a time-resolved fluorescence resonance energy resonance energy transfer-based assay and a label-free resonant waveguide grating-based assay was optimized for high-throughput screening formats.

Indexed as

HIVHTSlabel-free technologyNefprotein-protein interaction inhibitorresonant waveguide gratingSH3TR-FRET.

Identifiers

PMID24396731
PMCPMC3854662

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.