ArticleBiomicrofluidics2013
Effects of shear stresses and antioxidant concentrations on the production of reactive oxygen species in lung cancer cells.
Article in Biomicrofluidics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Advances in Bio-Microelectromechanical System-Based Sensors for Next-Generation Healthcare Applications.ACS omega · 2025Review
- Integrating computational fluid dynamics into organ-on-chip systems: a glioblastoma-centred design and validation framework.Frontiers in bioengineering and biotechnology · 2025Review
- Mechanical shear flow regulates the malignancy of colorectal cancer cells.The Kaohsiung journal of medical sciences · 2024Article
- A tempo-spatial controllable microfluidic shear-stress generator for in-vitro mimicking of the thrombus.Journal of nanobiotechnology · 2024Article
- Sesquiterpene-evoked phytochemical toxicity in PC12 neuronal cells reveals a variable degree of oxidative stress and alpha-tocopherol and glutathione-dependent protection.Current research in toxicology · 2024Article
- Fluid shear stress regulates the survival of circulating tumor cells via nuclear expansion.Journal of cell science · 2022Article
- Review
- Computational Simulations in Advanced Microfluidic Devices: A Review.Micromachines · 2021Review
- Fluid Shear Stress Induces EMT of Circulating Tumor Cells via JNK Signaling in Favor of Their Survival during Hematogenous Dissemination.International journal of molecular sciences · 2020Article
- A microfluidics-based wound-healing assay for studying the effects of shear stresses, wound widths, and chemicals on the wound-healing process.Scientific reports · 2019Article
- Mechanics and Actomyosin-Dependent Survival/Chemoresistance of Suspended Tumor Cells in Shear Flow.Biophysical journal · 2019Article
- Design and Fabrication of a Microfluidic Viscometer Based on Electrofluidic Circuits.Micromachines · 2018Article
- Cyclooxygenase-2 Inhibition Limits Angiotensin II-Induced DNA Oxidation and Protein Nitration in Humans.Frontiers in physiology · 2017Article
- Integration of Curved D-Type Optical Fiber Sensor with Microfluidic Chip.Sensors (Basel, Switzerland) · 2016Article
- Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli.Journal of visualized experiments : JoVE · 2016Article
- Comparison of Chip Inlet Geometry in Microfluidic Devices for Cell Studies.Molecules (Basel, Switzerland) · 2016Article
- Hypoxia and free radicals: role in tumor progression and the use of engineering-based platforms to address these relationships.Free radical biology & medicine · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reactive oxygen species (ROS) are known to be a key factor in the development of cancer, and many exogenous sources are supposed to be related to the formation of ROS. In this paper, a microfluidic chip was developed for studying the production of ROS in lung cancer cells under different chemical and physical stimuli. This chip has two unique features: (1) five relative concentrations of 0, 1/8, 1/2, 7/8, and 1 are achieved in the culture regions; (2) a shear stress gradient is produced inside each of the five culture areas. Lung cancer cells were seeded inside this biocompatible chip for investigating their response to different concentrations of H2O2, a chemical stimulus known to increase the production of ROS. Then the effect of shear stress, a physical stimulus, on lung cancer cells was examined, showing that the production of ROS was increased in response to a larger shear stress. Finally, two antioxidants, α-tocopherol and ferulic acid, were used to study their effects on reducing ROS. It was found that high-dose α-tocopherol was not able to effectively eliminate the ROS produced inside cells. This counter effect was not observed in cells cultured in a traditional chamber slide, where no shear stress was present. This result suggests that the current microfluidic chip provides an in vitro platform best mimicking the physiological condition where cells are under circulating conditions.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.