ArticleJournal of clinical laboratory analysis2014
Restriction analysis of β-tubulin gene for differentiation of the common pathogenic dermatophytes.
Article in Journal of clinical laboratory analysis, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Comparative Description of Dermatophyte Genomes: A State-of-the-Art Review.Mycopathologia · 2023Pooled it
- Addressing filamentous fungi-related onychomycosis in the era of antifungal resistance: assessment ofCurrent medical mycology · 2025Article
- Article
- Epidemiology of Dermatophytosis in Tehran, Iran: A Ten-year Retrospective Study.Archives of Iranian medicine · 2022Article
- MALDI-TOF MS for the rapid identification and drug susceptibility testing of filamentous fungi.Experimental and therapeutic medicine · 2019Article
- LowAntimicrobial agents and chemotherapy · 2018Article
- Epidemiological status of dermatophytosis in Guilan, north of Iran.Current medical mycology · 2017Article
- Hyphal wall protein 1 gene: A potential marker for the identification of differentCurrent medical mycology · 2016Article
- Epidemiological Aspects of Dermatophytosis in Khuzestan, southwestern Iran, an Update.Mycopathologia · 2016Article
- Genetic and Morphological Diversity of the Genus Penicillium From Mazandaran and Tehran Provinces, Iran.Jundishapur journal of microbiology · 2016Article
- A Case of Fungus Ball-Type Pansinusitis Due to Fusarium proliferatum.Mycopathologia · 2015Article
- A Molecular Epidemiological Survey of Clinically Important Dermatophytes in Iran Based on Specific RFLP Profiles of Beta-tubulin Gene.Iranian journal of public health · 2013Article
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6 authors.
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Abstract
backgroundIdentification of dermatophytes at the species level, relying on macro- and microscopic properties of the colonies is time-consuming, questioned in many circumstances, and requires considerable expertise. In this study, we examined the potency of a new genetic marker, β-tubulin (BT2) gene, for differentiation of dermatophytes in an in silico and experimental restriction fragment length polymorphism (RFLP) profile.
methodsThe BT2 sequences of dermatophyte species were retrieved from GenBank and analyzed using bioinformatics softwares to choose suitable restriction enzyme(s). Forty reference culture collections and 100 clinical isolates were PCR-amplified using the primers T1 and Bt2b and consequently subjected to virtual RFLP analysis. The dermatophytes were identified according to specific lengths of bands in agarose gel electrophoresis.
resultsAfter digestion of partially amplified β-tubulin gene with the restriction enzyme FatI, three dermatophyte species, that is, Microsporum gypseum, M. canis, and Trichophyton verrucosum yielded unique restriction maps while the remaining species including T. interdigitale, T. rubrum, T. tonsurans, T. schoenleinii, and T. violaceum, were identified by further restriction digestion by Alw21I, MwoI, and HpyCH4V endonucleases. The length of RFLP products was same as of those expected by computer analysis.
conclusionThe two-step BT2 restriction mapping used in this study is an effective tool for reliable differentiation of the clinically relevant species of dermatophytes.
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