ArticleMethods in molecular biology (Clifton, N.J.)2014
Cell-free translation of biofuel enzymes.
Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
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- Identification and characterization of a set of monocot BAHD monolignol transferases.Plant physiology · 2022Article
- pHBMT1, a BAHD-family monolignol acyltransferase, mediates lignin acylation in poplar.Plant physiology · 2022Article
- Function-driven single-cell genomics uncovers cellulose-degrading bacteria from the rare biosphere.The ISME journal · 2020Article
- Function-driven single-cell genomics uncovers cellulose-degrading bacteria from the rare biosphere.The ISME journal · 2020Article
- Accelerating the Production of Druggable Targets: Eukaryotic Cell-Free Systems Come into Focus.Methods and protocols · 2019Article
- Extent and Origins of Functional Diversity in a Subfamily of Glycoside Hydrolases.Journal of molecular biology · 2019Article
- Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling.Biotechnology for biofuels · 2017Article
- Enzymatic diversity of the Clostridium thermocellum cellulosome is crucial for the degradation of crystalline cellulose and plant biomass.Scientific reports · 2016Article
- Active site and laminarin binding in glycoside hydrolase family 55.The Journal of biological chemistry · 2015Article
- Multifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules.Biotechnology for biofuels · 2015Article
- Use of Nanostructure-Initiator Mass Spectrometry to Deduce Selectivity of Reaction in Glycoside Hydrolases.Frontiers in bioengineering and biotechnology · 2015Article
- Development of a High Throughput Platform for Screening Glycoside Hydrolases Based on Oxime-NIMS.Frontiers in bioengineering and biotechnology · 2015Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
In nature, bacteria and fungi are able to utilize recalcitrant plant materials by secreting a diverse set of enzymes. While genomic sequencing efforts offer exhaustive lists of genes annotated as potential polysaccharide-degrading enzymes, biochemical and functional characterizations of the encoded proteins are still needed to realize the full potential of this natural genomic diversity. This chapter outlines an application of wheat germ cell-free translation to the study of biofuel enzymes using genes from Clostridium thermocellum, a model cellulolytic organism. Since wheat germ extract lacks enzymatic activities that can hydrolyze insoluble polysaccharide substrates and is likewise devoid of enzymes that consume the soluble sugar products, the cell-free translation reactions provide a clean background for production and study of the reactions of biofuel enzymes. Examples of assays performed with individual enzymes or with small sets of enzymes obtained directly from cell-free translation are provided.
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Registered trials
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