ArticleGenetics2014
Neprilysins: an evolutionarily conserved family of metalloproteases that play important roles in reproduction in Drosophila.
Article in Genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 65 citations in OpenAlex.
- A novel female reproductive organ differentiates insemination and postmating responses in bedbugs.Molecular biology and evolution · 2026Article
- Dopamine neuron specific RNA-sequencing reveals Neprilysin 1 acts downstream of the cohesin complex to suppress learning.Communications biology · 2026Article
- Identification ofmicroPublication biology · 2026Article
- Neprilysin 4 controls acrosome structure and male fertility in Drosophila melanogaster.Communications biology · 2025Article
- Phylogenetic Relatedness Does not Predict Patterns of Parallel Transcriptome Adaptation in Drosophila.Genome biology and evolution · 2025Article
- How to Reproduce in the Siberian Winter: Proteome Dynamics Reveals the Timing of Reproduction-Related Processes in an Amphipod Species Endemic to Lake Baikal.Ecology and evolution · 2025Article
- Article
- Multi-omic approach to characterize the venom of the parasitic wasp Cotesia congregata (Hymenoptera: Braconidae).BMC genomics · 2025Article
- Neprilysin-Mediated Amyloid Beta Clearance and Its Therapeutic Implications in Neurodegenerative Disorders.ACS pharmacology & translational science · 2024Review
- Gene therapy: an alternative to treat Alzheimer's disease.Naunyn-Schmiedeberg's archives of pharmacology · 2024Review
- Immune system modulation & virus transmission during parasitism identified by multi-species transcriptomics of a declining insect biocontrol system.BMC genomics · 2024Article
- Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq ofeLife · 2023Article
- Octopaminergic/tyraminergic Tdc2 neurons regulate biased sperm usage in female Drosophila melanogaster.Genetics · 2022Article
- Proteolysis in Reproduction: Lessons From Gene-Modified Organism Studies.Frontiers in endocrinology · 2022Review
- Susceptibility of the Non-Targeted CrustaceanGenes · 2021Article
- Genome of the pincer wasp Gonatopus flavifemur reveals unique venom evolution and a dual adaptation to parasitism and predation.BMC biology · 2021Article
- Identification and bioinformatic analysis of neprilysin and neprilysin-like metalloendopeptidases inmicroPublication biology · 2021Article
- The Drosophila melanogaster Neprilysin Nepl15 is involved in lipid and carbohydrate storage.Scientific reports · 2021Article
- Article
- Proteomic Mapping of Multifunctional Complexes Within Triatomine Saliva.Frontiers in cellular and infection microbiology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Members of the M13 class of metalloproteases have been implicated in diseases and in reproductive fitness. Nevertheless, their physiological role remains poorly understood. To obtain a tractable model with which to analyze this protein family's function, we characterized the gene family in Drosophila melanogaster and focused on reproductive phenotypes. The D. melanogaster genome contains 24 M13 class protease homologs, some of which are orthologs of human proteases, including neprilysin. Many are expressed in the reproductive tracts of either sex. Using RNAi we individually targeted the five Nep genes most closely related to vertebrate neprilysin, Nep1-5, to investigate their roles in reproduction. A reduction in Nep1, Nep2, or Nep4 expression in females reduced egg laying. Nep1 and Nep2 are required in the CNS and the spermathecae for wild-type fecundity. Females that are null for Nep2 also show defects as hosts of sperm competition as well as an increased rate of depletion for stored sperm. Furthermore, eggs laid by Nep2 mutant females are fertilized normally, but arrest early in embryonic development. In the male, only Nep1 was required to induce normal patterns of female egg laying. Reduction in the expression of Nep2-5 in the male did not cause any dramatic effects on reproductive fitness, which suggests that these genes are either nonessential for male fertility or perform redundant functions. Our results suggest that, consistent with the functions of neprilysins in mammals, these proteins are also required for reproduction in Drosophila, opening up this model system for further functional analysis of this protein class and their substrates.
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