ArticleLaboratory investigation; a journal of technical methods and pathology2014
In vitro 3D angiogenesis assay in egg white matrix: comparison to Matrigel, compatibility to various species, and suitability for drug testing.
Article in Laboratory investigation; a journal of technical methods and pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- From Nutrition to Innovation: Biomedical Applications of Egg Components.Molecules (Basel, Switzerland) · 2025Review
- Standardization to Characterize the Complexity of Vessel Network Using the Aortic Ring Model.International journal of molecular sciences · 2024Article
- Egg White Photocrosslinkable Hydrogels as Versatile Bioinks for Advanced Tissue Engineering Applications.Advanced functional materials · 2024Article
- The Chicken Egg: An Advanced Material for Tissue Engineering.Biomolecules · 2024Review
- Cryogenic 3D Printing of GelMA/Graphene Bioinks: Improved Mechanical Strength and Structural Properties for Tissue Engineering.International journal of nanomedicine · 2024Article
- Article
- Egg White Alginate as a Novel Scaffold Biomaterial for 3D Salivary Cell Culturing.Biomimetics (Basel, Switzerland) · 2021Article
- Recent Advances in 3D Printing with Protein-Based Inks.Progress in polymer science · 2021Article
- The Optimization of a Novel Hydrogel-Egg White-Alginate for 2.5D Tissue Engineering of Salivary Spheroid-Like Structure.Molecules (Basel, Switzerland) · 2020Article
- 3D Cell Culture of Human Salivary Glands Using Nature-Inspired Functional Biomaterials: The Egg Yolk Plasma and Egg White.Materials (Basel, Switzerland) · 2020Article
- 3D Cultures of Salivary Gland Cells in Native or Gelled Egg Yolk Plasma, Combined with Egg White and 3D-Printing of Gelled Egg Yolk Plasma.Materials (Basel, Switzerland) · 2019Article
- A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues.Advanced healthcare materials · 2019Article
- Cancer stem cells contribute to angiogenesis and lymphangiogenesis in serous adenocarcinoma of the ovary.Angiogenesis · 2019Article
- Identification of Angiogenesis Inhibitors Using a Co-culture Cell Model in a High-Content and High-Throughput Screening Platform.SLAS technology · 2018Article
- Scaffold-Hopping of Aurones: 2-Arylideneimidazo[1,2-ACS medicinal chemistry letters · 2016Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro angiogenesis assays are commonly used to assess pro- or anti-angiogenic drug properties. Extracellular matrix (ECM) substitutes such as Matrigel and collagen gel became very popular in in vitro 3D angiogenesis assays as they enable tubule formation by endothelial cells from culture or aortic rings. However, these assays are usually used with a single cell type, lacking the complex cellular interactions occurring during angiogenesis. Here, we report a novel angiogenesis assay using egg white as ECM substitute. We found that, similar to Matrigel, egg white elicited prevascular network formation by endothelial and/or smooth muscle cell coculture. This matrix was suitable for various cells from human, mouse, and rat origin. It is compatible with aortic ring assay and also enables vascular and tumor cell coculture. Through simple labeling (DAPI, Hoechst 33258), cell location and resulting prevascular network formation can easily be quantified. Cell transfection with green fluorescent protein improved whole cell visualization and 3D structure characterization. Finally, egg-based assay dedicated to angiogenesis studies represents a reliable and cost-effective way to produce and analyze data regarding drug effects on vascular cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.