ArticleThe Histochemical journal1987
Light microscopical detection of antigens and lectin binding sites with gold-labelled reagents on semi-thin Lowicryl K4M sections: usefulness of the photochemical silver reaction for signal amplification.
Article in The Histochemical journal, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Lectins: a primer for histochemists and cell biologists.Histochemistry and cell biology · 2017Review
- Improvement of supersensitive immunohistochemistry with an autostainer: a simplified catalysed signal amplification system.The Histochemical journal · 2002Article
- Silver development in microscopy and bioanalysis: a new versatile formulation for modern needs.The Histochemical journal · 1998Article
- Applications of immunogold and lectin-gold labeling in tumor research and diagnosis.Histochemistry and cell biology · 1996Review
- Protein glycosylation in the endoplasmic reticulum and the Golgi apparatus and cell type-specificity of cell surface glycoconjugate expression: analysis by the protein A-gold and lectin-gold techniques.Histochemistry and cell biology · 1996Review
- The silver anniversary of gold: 25 years of the colloidal gold marker system for immunocytochemistry and histochemistry.Histochemistry and cell biology · 1996Review
- Subcellular distribution of tissue kallikrein and Na,K-ATPase alpha-subunit in rat parotid striated duct cells.Cell and tissue research · 1994Article
- Histochemistry of carbohydrates as performed by physical development procedures.The Histochemical journal · 1993Review
- Quantitative immunogold localization of Na, K-ATPase along rat nephron.Histochemistry · 1992Article
- A double protein A-gold-silver staining method for tissue antigens in light microscopy.The Histochemical journal · 1992Article
- Binding of botulinum neurotoxin to pure cholinergic nerve terminals isolated from the electric organ of Torpedo.Journal of neural transmission. General section · 1992Article
- Nuclear location of phosphoglycerate mutase BB isozyme in rat tissues.Histochemistry · 1992Article
- An efficient histochemical method for deoxyribonucleic acids using a silver enhancement procedure.The Histochemical journal · 1990Article
- Lectin--digoxigenin conjugates: a new hapten system for glycoconjugate cytochemistry.Histochemistry · 1990Article
- Article
- Light and electron microscopic demonstration of extracellular immunoglobulin deposition in renal tissue.Journal of clinical pathology · 1989Article
- Light and electron microscopic detection of (3 Gal beta 1,4 GlcNAc beta 1) sequences in asparagine-linked oligosaccharides with the Datura stramonium lectin.Histochemistry · 1989Article
- Light and electron microscopic detection of lectin binding sites in normal and disordered human gastric tissues.Journal of Tongji Medical University = Tong ji yi ke da xue xue bao · 1989Article
- Blastemal cells of nephroblastomatosis complex share an onco-developmental antigen with embryonic kidney and Wilms' tumor. An immunohistochemical study on polysialic acid distribution.The American journal of pathology · 1988Article
- Presence of the long chain form of polysialic acid of the neural cell adhesion molecule in Wilms' tumor. Identification of a cell adhesion molecule as an oncodevelopmental antigen and implications for tumor histogenesis.The American journal of pathology · 1988Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study, we have investigated the applicability of semi-thin sections from low temperature Lowicryl K4M-embedded tissues for cytochemical labelling with protein A-gold and lectin-gold complexes. In order to ensure the best possible signal-to-noise ratio antibodies, protein A-gold and lectin-gold were applied in concentrations used for labelling at the electron microscope level. Furthermore, due to the lack of an appropriate chemical procedure for resin removal, untreated semi-thin sections were incubated. Under such conditions, semi-thin sections displayed either no visible staining or only a faint incomplete staining. However, following photochemical silver reaction, the latent or faint incomplete staining was rendered visible in most cases. It is concluded that the same block of Lowicryl K4M-embedded tissue and the same labelling reagents can be used for both light and electron microscopical cytochemical studies. At the light microscopical level, a high degree of structural and specific staining information is obtained. The reactivity of cellular components with antibodies or lectins is preserved even after years of storage of the blocks or slides containing semi-thin sections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.