ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2014
Mitochondrial staining allows robust elimination of apoptotic and damaged cells during cell sorting.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systematic review and meta-analysis of cryopreserved bovine sperm assessment: harnessing imaging flow cytometry for multi-parametric analysis.Frontiers in veterinary science · 2024Pooled it
- Employment of the fluorescent probe hydroxy-naphthyl-methylbenzoxazole-based dye and its combination with fluorescent silica nanoparticles as potential heavy metal-targeting systems: results and troubleshooting in Cd-polluted environments.Analytical and bioanalytical chemistry · 2026Article
- Formulation of hispidulin-loaded sodium carboxymethyl cellulose nanoparticles: characterisation, biological activities and molecular insights.Frontiers in pharmacology · 2026Article
- Mitochondrial Metabolomics in Cancer: Mass Spectrometry-Based Approaches for Metabolic Rewiring Analysis and Therapeutic Discovery.Metabolites · 2025Review
- Predicting mitochondrial fission, fusion and depolarisation event locations from a single z-stack.PloS one · 2023Article
- NLR family pyrin domain containing 3 (NLRP3) and caspase 1 (CASP1) modulation by intracellular ClImmunology · 2021Article
- Article
- Mitochondrial isolation: when size matters.Wellcome open research · 2020Review
- Role of mitochondria in rescuing glycolytically inhibited subpopulation of triple negative but not hormone-responsive breast cancer cells.Scientific reports · 2019Article
- Article
- Intracellular transport is accelerated in early apoptotic cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Temporal Heterogeneity Metrics in Apoptosis Induced by Anticancer Drugs.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
High-speed fluorescence-activated cell sorting is relevant for a plethora of applications, such as PCR-based techniques, microarrays, cloning, and propagation of selected cell populations. We suggest a simple cell-sorting technique to eliminate early and late apoptotic and necrotic cells, with good signal-to-noise ratio and a high-purity yield. The mitochondrial potential dye, TMRE (tetramethylrhodamine ethyl ester perchlorate), was used to separate viable and non-apoptotic cells from the cell sorting samples. TMRE staining is reversible and does not affect cell proliferation and viability. Sorted TMRE(+) cells contained a negligible percentage of apoptotic and damaged cells and had a higher proliferative potential as compared with their counterpart cells, sorted on the basis of staining with DNA viability dye. This novel sorting technique using TMRE does not interfere with subsequent functional assays and is a method of choice for the enrichment of functionally active, unbiased cell populations.
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Registered trials
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