Evidence map›Paper›PMID 24394447›Full record

ArticleInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases2014

Retrotransposon OV-RTE-1 from the carcinogenic liver fluke Opisthorchis viverrini: potential target for DNA-based diagnosis.

Luyen Thi Phung, Alex Loukas, Paul J Brindley, Banchob Sripa, Thewarach Laha

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 3 countries.

Luyen Thi PhungDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Alex LoukasCentre for Biodiscovery and Molecular Development of Therapeutics, Queensland Tropical Health Alliance, James Cook University, Cairns, Queensland 4878, Australia.
Paul J BrindleyDepartment of Microbiology, Immunology and Tropical Medicine, and Research Center for Neglected Tropical and Infectious Diseases of Poverty, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.
Banchob SripaTropical Disease Research Laboratory, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Thewarach LahaDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: thewa_la@kku.ac.th.
Khon Kaen University · THGeorge Washington University · USJames Cook University · AU

Funding

Pathogenesis of Opisthorchiasis induced persistent periductal fibrosis and CCAP50AI098639 · NIAID · KHON KAEN UNIVERSITY · PI THINKHAMROP, BANDIT · 2012 to 2016
$1.6M
NIAID NIH HHS P50 AI098639
6 · The paper itself

Abstract

Infections by the fish-borne liver flukes Opisthorchis viverrini and Clonorchis sinensis can lead to bile duct cancer. These neglected tropical disease pathogens occur in East Asia, with O. viverrini primarily in Thailand and Laos and C. sinensis in Cambodia, Vietnam, and China. Genomic information about these pathogens holds the potential to improve disease treatment and control. Transcriptome analysis indicates that mobile genetic elements are active in O. viverrini, including a novel non-Long Terminal Repeat (LTR) retrotransposon. A consensus sequence of this element, termed OV-RTE-1, was assembled from expressed sequence tags and PCR amplified genomic DNA. OV-RTE-1 was 3330 bp in length, encoded 1101 amino acid residues and exhibited hallmark structures and sequences of non-LTR retrotransposons including a single open reading frame encoding apurinic-apyrimidinic endonuclease (EN) and reverse transcriptase (RT). Phylogenetic analyses confirmed that OV-RTE-1 was member of the RTE clade of non-LTR retrotransposons. OV-RTE-1 is the first non-LTR retrotransposon characterized from the trematode family Opisthorchiidae. Sequences of OV-RTE-1 were targeted to develop a diagnostic tool for detection of infection by O. viverrini. PCR specific primers for detection of O. viverrini DNA showed 100% specificity and sensitivity for detection of as little as 5 fg of O. viverrini DNA whereas the PCR based approach showed 62% sensitivity and 100% specificity with clinical stool samples. The OV-RTE-1 specific PCR could be developed as a molecular diagnostic for Opisthorchis infection targeting parasite eggs in stool samples, especially in regions of mixed infection of O. viverrini and/or C. sinensis and minute intestinal flukes.

Indexed as

RetroelementsAnimalsDNA-(Apurinic or Apyrimidinic Site) LyaseDNA, HelminthEvolution, MolecularFecesHelminth ProteinsHumansOpisthorchiasisOpisthorchisPhylogenyPolymerase Chain ReactionRNA-Directed DNA PolymeraseSensitivity and SpecificityTranscriptomeDNA-(Apurinic or Apyrimidinic Site) LyaseDNA, HelminthHelminth ProteinsRetroelementsRNA-Directed DNA PolymeraseDiagnosisFecesLiver flukeOpisthorchis viverriniRetrotransposon

Identifiers

PMID24394447
PMCPMC3955739
OpenAlexW1976606753

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.