ArticleMolecular therapy : the journal of the American Society of Gene Therapy2014
Conditioning the cochlea to facilitate survival and integration of exogenous cells into the auditory epithelium.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Induced Pluripotent Stem Cells, a Stepping Stone to In Vitro Human Models of Hearing Loss.Cells · 2022Review
- Article
- Photobiomodulation with a wavelength > 800 nm induces morphological changes in stem cells within otic organoids and scala media of the cochlea.Lasers in medical science · 2021Article
- Junctional Modulation of Round Window Membrane Enhances Dexamethasone Uptake into the Inner Ear and Recovery after NIHL.International journal of molecular sciences · 2021Article
- A human induced pluripotent stem cell-based modular platform to challenge sensorineural hearing loss.Stem cells (Dayton, Ohio) · 2021Review
- Research progress on flat epithelium of the inner ear.Physiological research · 2020Review
- Stem cell-based approaches: Possible route to hearing restoration?World journal of stem cells · 2020Review
- Using Sox2 to alleviate the hallmarks of age-related hearing loss.Ageing research reviews · 2020Review
- Stem Cell-Based Therapeutic Approaches to Restore Sensorineural Hearing Loss in Mammals.Neural plasticity · 2020Review
- Engraftment of Human Stem Cell-Derived Otic Progenitors in the Damaged Cochlea.Molecular therapy : the journal of the American Society of Gene Therapy · 2019Article
- Photobiomodulation promotes adenoviral gene transduction in auditory cells.Lasers in medical science · 2019Article
- Outlook and future of inner ear therapy.Hearing research · 2018Review
- Differentiation and transplantation of human induced pluripotent stem cell-derived otic epithelial progenitors in mouse cochlea.Stem cell research & therapy · 2018Article
- Potential of Gene and Cell Therapy for Inner Ear Hair Cells.BioMed research international · 2018Review
- Severe streptomycin ototoxicity in the mouse utricle leads to a flat epithelium but the peripheral neural degeneration is delayed.Hearing research · 2017Article
- Survival of human embryonic stem cells implanted in the guinea pig auditory epithelium.Scientific reports · 2017Article
- Genetic Correction of Induced Pluripotent Stem Cells From a Deaf Patient With MYO7A Mutation Results in Morphologic and Functional Recovery of the Derived Hair Cell-Like Cells.Stem cells translational medicine · 2016Article
- Stem Cell Therapy for Sensorineural Hearing Loss, Still Alive?Journal of audiology & otology · 2015Review
- Nonviral Reprogramming of Human Wharton's Jelly Cells Reveals Differences Between ATOH1 Homologues.Tissue engineering. Part A · 2015Article
- Ototoxicity-induced loss of hearing and inner hair cells is attenuated by HSP70 gene transfer.Molecular therapy. Methods & clinical development · 2015Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
The mammalian auditory epithelium (AE) cannot replace supporting cells and hair cells once they are lost. Therefore, sensorineural hearing loss associated with missing cells is permanent. This inability to regenerate critical cell types makes the AE a potential target for cell replacement therapies such as stem cell transplantation. Inserting stem cells into the AE of deaf ears is a complicated task due to the hostile, high potassium environment of the scala media in the cochlea, and the robust junctional complexes between cells in the AE that resist stem cell integration. Here, we evaluate whether temporarily reducing potassium levels in the scala media and disrupting the junctions in the AE make the cochlear environment more receptive and facilitate survival and integration of transplanted cells. We used sodium caprate to transiently disrupt the AE junctions, replaced endolymph with perilymph, and blocked stria vascularis pumps with furosemide. We determined that these three steps facilitated survival of HeLa cells in the scala media for at least 7 days and that some of the implanted cells formed a junctional contact with native AE cells. The data suggest that manipulation of the cochlear environment facilitates survival and integration of exogenously transplanted HeLa cells in the scala media.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.