ArticleMolecular therapy : the journal of the American Society of Gene Therapy2014
Skin electroporation of a plasmid encoding hCAP-18/LL-37 host defense peptide promotes wound healing.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 79 citations in OpenAlex.
- Systematic review and meta-analysis of mouse models of diabetes-associated ulcers.BMJ open diabetes research & care · 2020Pooled it
- Evaluation of LL-37 in healing of hard-to-heal venous leg ulcers: A multicentric prospective randomized placebo-controlled clinical trial.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2021Trial
- Antimicrobial Peptides for Diabetic Foot Ulcers and Infections: Current Evidence and Translational Perspectives.International journal of molecular sciences · 2026Review
- Cathelicidin LL37-loaded extracellular vesicles from Edwardsiella piscicida promote antibacterial and wound-healing activity.Scientific reports · 2025Article
- Antimicrobial peptide delivery to lung as peptibody mRNA in anti-inflammatory lipids treats multidrug-resistant bacterial pneumonia.Nature biotechnology · 2025Article
- Antimicrobial Peptides for Skin Wound Healing.Biomolecules · 2025Review
- Cathelicidins: Opportunities and Challenges in Skin Therapeutics and Clinical Translation.Antibiotics (Basel, Switzerland) · 2024Review
- In Vivo Wound Healing Model for Characterization of Gene Electrotransfer Effects in Mouse Skin.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Application of pulsed electric field technology to skin engineering.Frontiers in bioengineering and biotechnology · 2024Review
- Discovery of novel antibacterial agent for the infected wound treatment: all-hydrocarbon stapling optimization of LL-37.Theranostics · 2024Article
- Skin barrier-inflammatory pathway is a driver of the psoriasis-atopic dermatitis transition.Frontiers in medicine · 2024Review
- Effect of Elaeagnus Angustifolia extract on in vitro wound healing of human dermal fibroblast cells.BMC research notes · 2023Article
- Neutrophil-derived cathelicidin promotes cerebral angiogenesis after ischemic stroke.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2023Article
- Improving Vascularization of Biomaterials for Skin and Bone Regeneration by Surface Modification: A Narrative Review on Experimental Research.Bioengineering (Basel, Switzerland) · 2022Review
- In vitro and in vivo correlation of skin and cellular responses to nucleic acid delivery.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2022Article
- The evolution of clinical guidelines for antimicrobial photodynamic therapy of skin.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2022Review
- Chitosan hydrogel encapsulated with LL-37 peptide promotes deep tissue injury healing in a mouse model.Military Medical Research · 2020Article
- 1α,25-dihydroxyvitamin DNanomedicine (London, England) · 2018Article
- Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing.International journal of nanomedicine · 2018Article
- Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice.PLoS neglected tropical diseases · 2017Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
Abstract
Host defense peptides, in particular LL-37, are emerging as potential therapeutics for promoting wound healing and inhibiting bacterial growth. However, effective delivery of the LL-37 peptide remains limiting. We hypothesized that skin-targeted electroporation of a plasmid encoding hCAP-18/LL-37 would promote the healing of wounds. The plasmid was efficiently delivered to full-thickness skin wounds by electroporation and it induced expression of LL-37 in the epithelium. It significantly accelerated reepithelialization of nondiabetic and diabetic wounds and caused a significant VEGFa and interleukin (IL)-6 induction. IL-6 was involved in LL-37-mediated keratinocyte migration in vitro and IL-6 neutralizing antibodies delivered to mice were able to suppress the wound healing activity of the hCAP-18/LL-37 plasmid. In a hindlimb ischemia model, electroporation of the hCAP-18/LL-37 plasmid increased blood perfusion, reduced muscular atrophy, and upregulated the angiogenic chemokines VEGFa and SDF-1a, and their receptors VEGF-R and CXCR-4. These findings demonstrate that a localized gene therapy with LL-37 is a promising approach for the treatment of wounds.
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Registered trials
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