Evidence map›Paper›PMID 24393808›Full record

ArticleMolecular psychiatry2014

RNA-sequencing of the brain transcriptome implicates dysregulation of neuroplasticity, circadian rhythms and GTPase binding in bipolar disorder.

N Akula, J Barb, X Jiang, J R Wendland, K H Choi, S K Sen, L Hou, D T W Chen, G Laje, K Johnson and 6 more

Open access · bronzeAbstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular psychiatry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
66citing papers in PubMed, 1 pooled it
5.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

66 citing papers in PubMed, 1 synthesis or guideline pooled it, 126 citations in OpenAlex.

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6 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 7 institutions in 2 countries.

N AkulaHuman Genetics Branch, National Institute of Mental Health Intramural Research Program, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
J BarbMathematical and Statistical Computing Laboratory, Center for Information Technology, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
X JiangHuman Genetics Branch, National Institute of Mental Health Intramural Research Program, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
J R WendlandHuman Genetics Branch, National Institute of Mental Health Intramural Research Program, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
K H ChoiDepartment of Psychiatry, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
S K SenGenetic Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.ORCID 000000025781721X
L HouHuman Genetics Branch, National Institute of Mental Health Intramural Research Program, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
D T W ChenHuman Genetics Branch, National Institute of Mental Health Intramural Research Program, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
G LajeHuman Genetics Branch, National Institute of Mental Health Intramural Research Program, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
K JohnsonBioinformatics Section, Information Technology & Bioinformatics Program, Division of Intramural Research, National Institute of Neurological Disorders & Stroke, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
B K LipskaHuman Brain Collection Core, Division of Intramural Research Programs, National Institute of Mental Health Intramural Research Program, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
J E KleinmanLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA.
H Corrada-BravoDepartment of Computer Science, Institute for Advanced Computer Studies and Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD, USA.
S Detera-WadleighHuman Genetics Branch, National Institute of Mental Health Intramural Research Program, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
P J MunsonMathematical and Statistical Computing Laboratory, Center for Information Technology, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
F J McMahonHuman Genetics Branch, National Institute of Mental Health Intramural Research Program, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
National Human Genome Research Institute · USCenter for Information Technology · USJohns Hopkins University · USNational Institute of Mental Health · CZUniformed Services University of the Health Sciences · USUnited States Department of Health and Human Services · USUniversity of Maryland, College Park · US

Funding

Procurement and Characterization of Postmortem Brain TissueZICMH002903 · NIMH · NATIONAL INSTITUTE OF MENTAL HEALTH · PI MARENCO, STEFANO · 2009 to 2025
$56.0M
Mapping genes that contribute to bipolar disorderZIAMH002810 · NIMH · NATIONAL INSTITUTE OF MENTAL HEALTH · PI MCMAHON, FRANCIS J · 2009 to 2025
$31.2M
Postmortem Brain Tissue Examination in Neuropsychiatric DisordersZIAMH002399 · NIMH · NATIONAL INSTITUTE OF MENTAL HEALTH · PI AMARA, SUSAN · 2009 to 2014
$11.9M
Statistical And Computational Methods For Gene Expression and Proteomic AnalysisZIACT000266 · CIT · CENTER FOR INFORMATION TECHNOLOGY · PI MUNSON, PETER J · 2009 to 2016
$4.2M
Bioinformatics and Analysis of Genomics High Throughput DataZIACT000277 · CIT · CENTER FOR INFORMATION TECHNOLOGY · PI BARB-SMITH, JENNIFER · 2014 to 2020
$1.2M
Intramural NIH HHS ZIA CT000277Intramural NIH HHS ZIA MH002810NIMH NIH HHS K99MH085098PHS HHS ZIAMH002810
6 · The paper itself

Abstract

RNA-sequencing (RNA-seq) is a powerful technique to investigate the complexity of gene expression in the human brain. We used RNA-seq to survey the brain transcriptome in high-quality postmortem dorsolateral prefrontal cortex from 11 individuals diagnosed with bipolar disorder (BD) and from 11 age- and gender-matched controls. Deep sequencing was performed, with over 350 million reads per specimen. At a false discovery rate of <5%, we detected five differentially expressed (DE) genes and 12 DE transcripts, most of which have not been previously implicated in BD. Among these, Prominin 1/CD133 and ATP-binding cassette-sub-family G-member2 (ABCG2) have important roles in neuroplasticity. We also show for the first time differential expression of long noncoding RNAs (lncRNAs) in BD. DE transcripts include those of serine/arginine-rich splicing factor 5 (SRSF5) and regulatory factor X4 (RFX4), which along with lncRNAs have a role in mammalian circadian rhythms. The DE genes were significantly enriched for several Gene Ontology categories. Of these, genes involved with GTPase binding were also enriched for BD-associated SNPs from previous genome-wide association studies, suggesting that differential expression of these genes is not simply a consequence of BD or its treatment. Many of these findings were replicated by microarray in an independent sample of 60 cases and controls. These results highlight common pathways for inherited and non-inherited influences on disease risk that may constitute good targets for novel therapies.

Indexed as

TranscriptomeAdultAgedBipolar DisorderCircadian RhythmFemaleGenome-Wide Association StudyGTP PhosphohydrolasesHumansMaleMeta-Analysis as TopicMicroarray AnalysisMiddle AgedNeuronal PlasticityPolymerase Chain ReactionPrefrontal CortexGTP Phosphohydrolases

Identifiers

PMID24393808
PMCPMC5560442
OpenAlexW1977521681

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.