ArticleMicrobial biotechnology2014
Xylose-fermenting Pichia stipitis by genome shuffling for improved ethanol production.
Article in Microbial biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 40 citations in OpenAlex.
- Applications and research advance of genome shuffling for industrial microbial strains improvement.World journal of microbiology & biotechnology · 2020Review
- Improvement ofFood science and biotechnology · 2018Article
- Genome and metabolic engineering in non-conventional yeasts: Current advances and applications.Synthetic and systems biotechnology · 2017Review
- Expression, Docking, and Molecular Dynamics of Endo-BioMed research international · 2017Article
- Improvement of Bacillus subtilis for poly-γ-glutamic acid production by genome shuffling.Microbial biotechnology · 2016Article
- Systematic development of biomass overproducingSynthetic and systems biotechnology · 2016Article
- Activating and Elucidating Metabolism of Complex Sugars in Yarrowia lipolytica.Applied and environmental microbiology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xylose fermentation is necessary for the bioconversion of lignocellulose to ethanol as fuel, but wild-type Saccharomyces cerevisiae strains cannot fully metabolize xylose. Several efforts have been made to obtain microbial strains with enhanced xylose fermentation. However, xylose fermentation remains a serious challenge because of the complexity of lignocellulosic biomass hydrolysates. Genome shuffling has been widely used for the rapid improvement of industrially important microbial strains. After two rounds of genome shuffling, a genetically stable, high-ethanol-producing strain was obtained. Designated as TJ2-3, this strain could ferment xylose and produce 1.5 times more ethanol than wild-type Pichia stipitis after fermentation for 96 h. The acridine orange and propidium iodide uptake assays showed that the maintenance of yeast cell membrane integrity is important for ethanol fermentation. This study highlights the importance of genome shuffling in P. stipitis as an effective method for enhancing the productivity of industrial strains.
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Registered trials
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