ArticleBMC complementary and alternative medicine2014
Discovery of potential anti-infectives against Staphylococcus aureus using a Caenorhabditis elegans infection model.
Article in BMC complementary and alternative medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 84 citations in OpenAlex.
- Article
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- Loratadine Combats Methicillin-ResistantACS infectious diseases · 2024Article
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- Development and Antibacterial Properties of 4-[4-(Anilinomethyl)-3-phenylpyrazol-1-yl]benzoic Acid Derivatives as Fatty Acid Biosynthesis Inhibitors.Journal of medicinal chemistry · 2023Article
- Review
- Mitigation of quorum sensing mediated virulence factors ofFrontiers in microbiology · 2023Article
- A Nonclassical Mechanism of β-Lactam Resistance in Methicillin-Resistant Staphylococcus aureus and Its Effect on Virulence.Microbiology spectrum · 2022Article
- The Prospects ofAntioxidants (Basel, Switzerland) · 2022Review
- A Multiwell-Plate Caenorhabditis elegans Assay for Assessing the Therapeutic Potential of Bacteriophages against Clinical Pathogens.Microbiology spectrum · 2022Article
- Evaluation ofFrontiers in pharmacology · 2022Article
- Article
- Bacterial Quality, Prevalence of Pathogens, and Molecular Characterization of Biofilm-ProducingAnimals : an open access journal from MDPI · 2021Article
- Heat-TreatedAntioxidants (Basel, Switzerland) · 2021Article
- Herbal-Based Formulation ContainingPharmaceuticals (Basel, Switzerland) · 2021Article
- In Vitro and In Vivo Antibacterial Activity of Gliotoxin Alone and in Combination with Antibiotics againstToxins · 2021Article
- Phenotypic Screening inPharmaceuticals (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe limited antibiotic options for effective control of methicillin-resistant Staphylococcus aureus infections has led to calls for new therapeutic approaches to combat this human pathogen. An alternative approach to control MRSA is through the use of anti-infective agents that selectively disrupt virulence-mediated pathways without affecting microbial cell viability or by modulating the host natural immune defenses to combat the pathogen.
methodsWe established a C. elegans - S. aureus liquid-based assay to screen for potential anti-infectives against S. aureus. The assay was utilized to screen 37 natural extracts and 29 synthetic compounds for the ability to extend the lifespan of infected nematodes. Disc diffusion and MIC microdilution tests were used to evaluate the anti-microbial properties of these natural extracts and synthetic compounds whilst in vivo bacterial CFU within the C. elegans gut were also enumerated.
resultsWe screened a total of 37 natural extracts and 29 synthetic compounds for anti-infective properties. The screen successfully revealed 14 natural extracts from six plants (Nypa fruticans, Swietenia macrophylla, Curcuma longa, Eurycoma longifolia, Orthosiphon stamineus and Silybum eburneum) and one marine sample (Faunus ater) that improved the survival of S. aureus-infected worms by at least 2.8-fold as well as 14 synthetic compounds that prolonged the survival of S. aureus-infected nematodes by 4-fold or greater. An anti-microbial screen of all positive hits demonstrated that 8/28 hits had no effect on S. aureus growth. Of these 8 candidates, 5 of them also protected the worms from MRSA infection. We also noted that worms exposed to N. fruticans root and O. stamineus leaf extracts showed reduced intestinal colonization by live S. aureus. This suggests that these extracts could possibly activate host immunity to eliminate the bacteria or interfere with factor/s that prevents pathogen accumulation.
conclusionWe have successfully demonstrated the utility of this liquid-based screen to identify anti-infective substances that prolong S. aureus-infected host survival without affecting bacterial cell viability.
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