ArticlePloS one2014
Label-free imaging of lipid depositions in C. elegans using third-harmonic generation microscopy.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Interface-Sensitive Epi-Coherent Anti-Stokes Raman Scattering Microscopy for Imaging Cell Adhesion Dynamics.Analytical chemistry · 2026Article
- Interface-Sensitive Epi-Coherent Anti-Stokes Raman Scattering Microscopy for Imaging Cell Adhesion Dynamics.bioRxiv : the preprint server for biology · 2025Article
- Article
- Label-free fluorescence microscopy: revisiting the opportunities with autofluorescent molecules and harmonic generations as biosensors and biomarkers for quantitative biology.Biophysical reviews · 2023Review
- Age-dependent nuclear lipid droplet deposition is a cellular hallmark of aging in Caenorhabditis elegans.Aging cell · 2023Article
- Multiscale spectroscopic analysis of lipids in dimorphic and oleaginous Mucor circinelloides accommodate sustainable targeted lipid production.Fungal biology and biotechnology · 2023Article
- Application ofInternational journal of molecular sciences · 2023Review
- Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopy.Npj imaging · 2023Article
- Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi.Scientific reports · 2022Article
- A human-origin probiotic cocktail ameliorates aging-related leaky gut and inflammation via modulating the microbiota/taurine/tight junction axis.JCI insight · 2020Article
- Neuroendocrine regulation of fat metabolism by autophagy gene atg-18 in C. elegans dauer larvae.FEBS open bio · 2019Article
- The DIVER Microscope for Imaging in Scattering Media.Methods and protocols · 2019Review
- Cancer Immunotherapy Getting Brainy: Visualizing the Distinctive CNS Metastatic Niche to Illuminate Therapeutic Resistance.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2017Review
- Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy.Scientific reports · 2017Article
- Ectopic fat deposition contributes to age-associated pathology in Caenorhabditis elegans.Journal of lipid research · 2017Article
- Third harmonic generation imaging for fast, label-free pathology of human brain tumors.Biomedical optics express · 2016Article
- Third harmonic generation microscopy of a mouse retina.Molecular vision · 2015Article
- Raman and coherent anti-Stokes Raman scattering microscopy studies of changes in lipid content and composition in hormone-treated breast and prostate cancer cells.Journal of biomedical optics · 2014Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elucidation of the molecular mechanisms regulating lipid storage and metabolism is essential for mitigating excess adiposity and obesity, which has been associated with increased prevalence of severe pathological conditions such as cardiovascular disorders and type II diabetes, worldwide. However, imaging fatty acid distribution and dynamics in vivo, at the cellular or organismal level is challenging. We developed a label-free method for visualizing lipid depositions in vivo, based on third harmonic generation (THG) microscopy. THG imaging requires a single pulsed-laser light source, alleviating the technical challenges of implementing coherent anti-Stokes Raman scattering spectroscopy (CARS) to detect fat stores in living cells. We demonstrate that THG can be used to efficiently and reliably visualize lipid droplets in Caenorhabditis elegans. Thus, THG microscopy offers a versatile alternative to fluorescence and dye-based approaches for lipid biology research.
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