ArticlePloS one2014
Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Human bone marrow-mesenchymal stem cells differentiation into brain-like endothelial cells.Turkish journal of biology = Turk biyoloji dergisi · 2026Article
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- Self-assembling 3D vessel-on-chip model with hiPSC-derived astrocytes.Stem cell reports · 2024Article
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- ZNF787 and HDAC1 Mediate Blood-Brain Barrier Permeability in an In Vitro Model of Alzheimer's Disease Microenvironment.Neurotoxicity research · 2024Article
- Characterization of an iPSC-based barrier model for blood-brain barrier investigations using the SBAD0201 stem cell line.Fluids and barriers of the CNS · 2023Article
- Modeling Brain Vasculature Immune Interactions In Vitro.Cold Spring Harbor perspectives in medicine · 2023Review
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- Targetability of the neurovascular unit in inflammatory diseases of the central nervous system.Immunological reviews · 2022Review
- Crosstalk of Astrocytes and Other Cells during Ischemic Stroke.Life (Basel, Switzerland) · 2022Review
- Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model.Cell reports · 2022Article
- Generation of a novel model of bioengineered human oral mucosa with increased vascularization potential.Journal of periodontal research · 2021Article
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- Physiological and Pathological Factors Affecting Drug Delivery to the Brain by Nanoparticles.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- TNFα priming through its interaction with TNFR2 enhances endothelial progenitor cell immunosuppressive effect: new hope for their widespread clinical application.Cell communication and signaling : CCS · 2021Article
- Advancing human induced pluripotent stem cell-derived blood-brain barrier models for studying immune cell interactions.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- The TNF/TNFR2 signaling pathway is a key regulatory factor in endothelial progenitor cell immunosuppressive effect.Cell communication and signaling : CCS · 2020Article
- Serum-derived factors of breast cancer patients with brain metastases alter permeability of a human blood-brain barrier model.Fluids and barriers of the CNS · 2020Article
- In vitro modeling of the neurovascular unit: advances in the field.Fluids and barriers of the CNS · 2020Review
- P-glycoprotein Expression Is Upregulated in a Pre-Clinical Model of Traumatic Brain Injury.Neurotrauma reports · 2020Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe vascular system is adapted to specific functions in different tissues and organs. Vascular endothelial cells are important elements of this adaptation, leading to the concept of 'specialized endothelial cells'. The phenotype of these cells is highly dependent on their specific microenvironment and when isolated and cultured, they lose their specific features after few passages, making models using such cells poorly predictive and irreproducible. We propose a new source of specialized endothelial cells based on cord blood circulating endothelial progenitors (EPCs). As prototype examples, we evaluated the capacity of EPCs to acquire properties characteristic of cerebral microvascular endothelial cells (blood-brain barrier (BBB)) or of arterial endothelial cells, in specific inducing culture conditions. APPROACH AND
resultsFirst, we demonstrated that EPC-derived endothelial cells (EPDCs) co-cultured with astrocytes acquired several BBB phenotypic characteristics, such as restricted paracellular diffusion of hydrophilic solutes and the expression of tight junction proteins. Second, we observed that culture of the same EPDCs in a high concentration of VEGF resulted, through activation of Notch signaling, in an increase of expression of most arterial endothelial markers.
conclusionsWe have thus demonstrated that in vitro culture of early passage human cord blood EPDCs under specific conditions can induce phenotypic changes towards BBB or arterial phenotypes, indicating that these EPDCs maintain enough plasticity to acquire characteristics of a variety of specialized phenotypes. We propose that this property of EPDCs might be exploited for producing specialized endothelial cells in culture to be used for drug testing and predictive in vitro assays.
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