ArticlePloS one2014
Identification of a novel gene signature of ES cells self-renewal fluctuation through system-wide analysis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Identification ofGenes · 2022Article
- DNA replication fork speed underlies cell fate changes and promotes reprogramming.Nature genetics · 2022Article
- Expanding the Differentiation Potential of Already-Established Pluripotent Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- CHIR99021, trough GSK-3β Targeting, Reduces Epithelioid Sarcoma Cell Proliferation by Activating Mitotic Catastrophe and Autophagy.International journal of molecular sciences · 2021Article
- Retinoic acid signaling is critical during the totipotency window in early mammalian development.Nature structural & molecular biology · 2021Article
- Oncostatin M Maintains Naïve Pluripotency of mESCs by Tetraploid Embryo Complementation (TEC) Assay.Frontiers in cell and developmental biology · 2021Article
- ZSCAN4EMBO reports · 2020Article
- A novel member of Prame family, Gm12794c, counteracts retinoic acid differentiation through the methyltransferase activity of PRC2.Cell death and differentiation · 2020Article
- Induced Pluripotent Stem Cells Reprogrammed with Three Inhibitors Show Accelerated Differentiation Potentials with High Levels of 2-Cell Stage Marker Expression.Stem cell reports · 2019Article
- Retinoic Acid Induces Embryonic Stem Cells (ESCs) Transition to 2 Cell-Like State Through a Coordinated Expression ofFrontiers in cell and developmental biology · 2019Article
- Extracellular Vesicles: A New Prospective in Crosstalk between Microenvironment and Stem Cells in Hematological Malignancies.Stem cells international · 2018Review
- Zscan4 Is Activated after Telomere Shortening in Mouse Embryonic Stem Cells.Stem cell reports · 2016Article
- Tcstv1 and Tcstv3 elongate telomeres of mouse ES cells.Scientific reports · 2016Article
- Article
- Systems biology analysis reveals NFAT5 as a novel biomarker and master regulator of inflammatory breast cancer.Journal of translational medicine · 2015Article
- The molecular underpinnings of totipotency.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic Stem cells (ESCs) can be differentiated into ectoderm, endoderm, and mesoderm derivatives, producing the majority of cell types. In regular culture conditions, ESCs' self-renewal is maintained through molecules that inhibit spontaneous differentiation enabling long-term cellular expansion. This undifferentiating condition is characterized by multiple metastable states that fluctuate between self-renewal and differentiation balance. Here, we aim to characterize the high-pluripotent ESC metastate marked by the expression of Zscan4 through a supervised machine learning framework based on an ensemble of support vector machine (SVM) classifiers. Our study revealed a leukaemia inhibitor factor (Lif) dependent not-canonical pluripotency signature (AF067063, BC061212, Dub1, Eif1a, Gm12794, Gm13871, Gm4340, Gm4850, Tcstv1/3, and Zfp352), that specifically marks Zscan4 ESCs' fluctuation. This novel ESC metastate is enhanced by high-pluripotency culture conditions obtained through Extracellular signal Regulated-Kinase (ERK) and Glycogen synthase kinase-3 (Gsk-3) signaling inhibition (2i). Significantly, we reported that the conditional ablation of the novel ESC metastate marked by the expression of Gm12794 is required for ESCs self-renewal maintenance. In conclusion, we extend the comprehension of ESCs biology through the identification of a novel molecular signature associated to pluripotency programming.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.