ArticlePloS one2013
The yeast p5 type ATPase, spf1, regulates manganese transport into the endoplasmic reticulum.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 68 citations in OpenAlex.
- Structural basis of an endoplasmic reticulum EMC:Spf1 insertase-dislocase complex.Nature structural & molecular biology · 2026Article
- Tail-anchored protein C-terminal domains stimulate ATP hydrolysis by the P5A-ATPase Spf1p.The Journal of biological chemistry · 2026Article
- Homology Modeling of Type-P5 ATPases from the Malaria Parasite: Insight into Their Functions and Evolution, and Implications About the Effect and Role of Intrinsically Disordered Protein Structure.Pathogens (Basel, Switzerland) · 2025Article
- The structure and function of P5A-ATPases.Nature communications · 2024Article
- Validation Study of Physical Activity and Sedentary Behavior in African-American Men and Women.Journal of racial and ethnic health disparities · 2024Article
- Structures and coordination chemistry of transporters involved in manganese and iron homeostasis.Biochemical Society transactions · 2023Review
- A genome-wide optical pooled screen reveals regulators of cellular antiviral responses.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Endoplasmic Reticulum and the Fidelity of Nascent Protein Localization.Cold Spring Harbor perspectives in biology · 2023Review
- The Endoplasmic Reticulum ATP13A1 is Essential for MAVS-Mediated Antiviral Innate Immunity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Impaired XK recycling for importing manganese underlies striatal vulnerability in Huntington's disease.The Journal of cell biology · 2022Article
- Functional single-cell genomics of human cytomegalovirus infection.Nature biotechnology · 2022Article
- The P5-type ATPase ATP13A1 modulates major histocompatibility complex I-related protein 1 (MR1)-mediated antigen presentation.The Journal of biological chemistry · 2022Article
- Cell death induction inCurrent research in microbial sciences · 2022Review
- Cancer associated mutations in Sec61γ alter the permeability of the ER translocase.PLoS genetics · 2021Article
- Fungal-Metal Interactions: A Review of Toxicity and Homeostasis.Journal of fungi (Basel, Switzerland) · 2021Review
- Potential Physiological Relevance of ERAD to the Biosynthesis of GPI-Anchored Proteins in Yeast.International journal of molecular sciences · 2021Review
- Highly exposed segment of the Spf1p P5A-ATPase near transmembrane M5 detected by limited proteolysis.PloS one · 2021Article
- The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.Science (New York, N.Y.) · 2020Article
- The P5-type ATPase Spf1 is required for development and virulence of the rice blast fungus Pyricularia oryzae.Current genetics · 2020Article
- The conserved C-terminus of Sss1p is required to maintain the endoplasmic reticulum permeability barrier.The Journal of biological chemistry · 2020Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The endoplasmic reticulum (ER) is a large, multifunctional and essential organelle. Despite intense research, the function of more than a third of ER proteins remains unknown even in the well-studied model organism Saccharomyces cerevisiae. One such protein is Spf1, which is a highly conserved, ER localized, putative P-type ATPase. Deletion of SPF1 causes a wide variety of phenotypes including severe ER stress suggesting that this protein is essential for the normal function of the ER. The closest homologue of Spf1 is the vacuolar P-type ATPase Ypk9 that influences Mn(2+) homeostasis. However in vitro reconstitution assays with Spf1 have not yielded insight into its transport specificity. Here we took an in vivo approach to detect the direct and indirect effects of deleting SPF1. We found a specific reduction in the luminal concentration of Mn(2+) in ∆spf1 cells and an increase following it's overexpression. In agreement with the observed loss of luminal Mn(2+) we could observe concurrent reduction in many Mn(2+)-related process in the ER lumen. Conversely, cytosolic Mn(2+)-dependent processes were increased. Together, these data support a role for Spf1p in Mn(2+) transport in the cell. We also demonstrate that the human sequence homologue, ATP13A1, is a functionally conserved orthologue. Since ATP13A1 is highly expressed in developing neuronal tissues and in the brain, this should help in the study of Mn(2+)-dependent neurological disorders.
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