ArticlePloS one2013
Knocking-down Meloidogyne incognita proteases by plant-delivered dsRNA has negative pleiotropic effect on nematode vigor.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Disentangling Arachis response to biotic and abiotic stress using multi-transcriptomics integration.BMC plant biology · 2026Article
- Biochemical and nanotechnological approaches to combat phytoparasitic nematodes.Plant biotechnology journal · 2024Review
- Silencing Ditylenchus destructor cathepsin L-like cysteine protease has negative pleiotropic effect on nematode ontogenesis.Scientific reports · 2024Article
- Assessment of nematicidal and plant growth-promoting effects ofFrontiers in plant science · 2023Article
- Transgenic Improvement for Biotic Resistance of Crops.International journal of molecular sciences · 2022Review
- Host-mediated RNAi for simultaneous silencing of different functional groups of genes in Meloidogyne incognita using fusion cassettes in Nicotiana tabacum.Plant cell reports · 2021Article
- Article
- A new fungus-mediated RNAi method established and used to study the fatty acid and retinol binding protein function of the plant-parasitic nematodeRNA biology · 2021Article
- RNA interference and crop protection against biotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Genome Expression Dynamics Reveal the Parasitism Regulatory Landscape of the Root-Knot NematodeGenes · 2021Article
- Cyst nematode bio-communication with plants: implications for novel management approaches.Pest management science · 2021Review
- Chitin Biosynthesis Inhibition ofInternational journal of molecular sciences · 2020Article
- Simultaneous RNAi Knockdown of Three FMRFamide-Like Peptide Genes,Frontiers in microbiology · 2020Article
- Attempt to Silence Genes of the RNAi Pathways of the Root-Knot Nematode,Frontiers in plant science · 2020Article
- Transcriptome analysis of Globodera pallida from the susceptible host Solanum tuberosum or the resistant plant Solanum sisymbriifolium.Scientific reports · 2019Article
- Host-induced silencing of Mi-msp-1 confers resistance to root-knot nematode Meloidogyne incognita in eggplant.Transgenic research · 2019Article
- Selenium Nanoparticles-an Inducer of Tomato Resistance to the Root-Knot Nematode Meloidogyne incognita (Kofoid et White, 1919) Chitwood 1949.Doklady. Biochemistry and biophysics · 2018Article
- Direct experimental manipulation of intestinal cells in Ascaris suum, with minor influences on the global transcriptome.International journal for parasitology · 2017Article
- Host Delivered RNAi of Two Cuticle Collagen Genes,Frontiers in plant science · 2017Article
- RNA Interference: A Novel Source of Resistance to Combat Plant Parasitic Nematodes.Frontiers in plant science · 2017Review
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The root-knot nematode Meloidogyne incognita causes serious damage and yield losses in numerous important crops worldwide. Analysis of the M. incognita genome revealed a vast number of proteases belonging to five different catalytic classes. Several reports indicate that M. incognita proteases could play important roles in nematode parasitism, besides their function in ordinary digestion of giant cell contents for feeding. The precise roles of these proteins during parasitism however are still unknown, making them interesting targets for gene silencing to address protein function. In this study we have knocked-down an aspartic (Mi-asp-1), a serine (Mi-ser-1) and a cysteine protease (Mi-cpl-1) by RNAi interference to get an insight into the function of these enzymes during a host/nematode interaction. Tobacco lines expressing dsRNA for Mi-ser-1 (dsSER), Mi-cpl-1 (dsCPL) and for the three genes together (dsFusion) were generated. Histological analysis of galls did not show clear differences in giant cell morphology. Interestingly, nematodes that infected plants expressing dsRNA for proteases produced a reduced number of eggs. In addition, nematode progeny matured in dsSER plants had reduced success in egg hatching, while progeny resulting from dsCPL and dsFusion plants were less successful to infect wild-type host plants. Quantitative PCR analysis confirmed a reduction in transcripts for Mi-cpl-1 and Mi-ser-1 proteases. Our results indicate that these proteases are possibly involved in different processes throughout nematode development, like nutrition, reproduction and embryogenesis. A better understanding of nematode proteases and their possible role during a plant-nematode interaction might help to develop new tools for phytonematode control.
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