ArticlePloS one2013
Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 45 citations in OpenAlex.
- Endothelial Cell-Pericyte Crosstalk in the Brain Balances Microvascular Stability and Plasticity.Arteriosclerosis, thrombosis, and vascular biology · 2026Review
- 3D endothelial network formation in hydrogels improved by stromal cells and specific growth factors.Scientific reports · 2025Article
- Defining the Functional Influence of Endothelial Cell-Expressed Oncogenic Activating Mutations on Vascular Morphogenesis and Capillary Assembly.The American journal of pathology · 2024Article
- Selective mural cell recruitment of pericytes to networks of assembling endothelial cell-lined tubes.Frontiers in cell and developmental biology · 2024Article
- Elucidating the Morphogenic and Signaling Roles of Defined Growth Factors Controlling Human Endothelial Cell Lumen Formation Versus Sprouting Behavior.The American journal of pathology · 2023Article
- Extracellular Matrix Regulation of Vascular Morphogenesis, Maturation, and Stabilization.Cold Spring Harbor perspectives in medicine · 2023Review
- Endothelial k-RasV12 Expression Induces Capillary Deficiency Attributable to Marked Tube Network Expansion Coupled to Reduced Pericytes and Basement Membranes.Arteriosclerosis, thrombosis, and vascular biology · 2022Article
- Proinflammatory mediators, TNFα, IFNγ, and thrombin, directly induce lymphatic capillary tube regression.Frontiers in cell and developmental biology · 2022Article
- Molecular basis for pericyte-induced capillary tube network assembly and maturation.Frontiers in cell and developmental biology · 2022Review
- Heterotypic Multicellular Spheroids as Experimental and Preclinical Models of Sprouting Angiogenesis.Biology · 2021Review
- Protective Effects of Collagen Tripeptides in Human Aortic Endothelial Cells by Restoring ROS-Induced Transcriptional Repression.Nutrients · 2021Article
- DNA Damage Baseline Predicts Resilience to Space Radiation and Radiotherapy.Cell reports · 2020Article
- Defining an Upstream VEGF (Vascular Endothelial Growth Factor) Priming Signature for Downstream Factor-Induced Endothelial Cell-Pericyte Tube Network Coassembly.Arteriosclerosis, thrombosis, and vascular biology · 2020Article
- Defining Endothelial Cell-Derived Factors That Promote Pericyte Recruitment and Capillary Network Assembly.Arteriosclerosis, thrombosis, and vascular biology · 2020Article
- Robust and Scalable Angiogenesis Assay of Perfused 3D Human iPSC-Derived Endothelium for Anti-Angiogenic Drug Screening.International journal of molecular sciences · 2020Article
- Article
- Biofabrication of thick vascularized neo-pedicle flaps for reconstructive surgery.Translational research : the journal of laboratory and clinical medicine · 2019Review
- Consensus guidelines for the use and interpretation of angiogenesis assays.Angiogenesis · 2018Review
- Src- and Fyn-dependent apical membrane trafficking events control endothelial lumen formation during vascular tube morphogenesis.PloS one · 2017Article
- Characterizing the angiogenic activity of patients with single ventricle physiology and aortopulmonary collateral vessels.The Journal of thoracic and cardiovascular surgery · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We describe a novel 3D fibrin matrix model using recombinant hematopoietic stem cell cytokines under serum-free defined conditions which promotes the assembly of human endothelial cell (EC) tubes with co-associated pericytes. Individual ECs and pericytes are randomly mixed together and EC tubes form that is accompanied by pericyte recruitment to the EC tube abluminal surface over a 3-5 day period. These morphogenic processes are stimulated by a combination of the hematopoietic stem cell cytokines, stem cell factor, interleukin-3, stromal derived factor-1α, and Flt-3 ligand which are added in conjunction with fibroblast growth factor (FGF)-2 into the fibrin matrix. In contrast, this tube morphogenic response does not occur under serum-free defined conditions when VEGF and FGF-2 are added together in the fibrin matrices. We recently demonstrated that VEGF and FGF-2 are able to prime EC tube morphogenic responses (i.e. added overnight prior to the morphogenic assay) to hematopoietic stem cell cytokines in collagen matrices and, interestingly, they also prime EC tube morphogenesis in 3D fibrin matrices. EC-pericyte interactions in 3D fibrin matrices leads to marked vascular basement membrane assembly as demonstrated using immunofluorescence and transmission electron microscopy. Furthermore, we show that hematopoietic stem cell cytokines and pericytes stimulate EC sprouting in fibrin matrices in a manner dependent on the α5β1 integrin. This novel co-culture system, under serum-free defined conditions, allows for a molecular analysis of EC tube assembly, pericyte recruitment and maturation events in a critical ECM environment (i.e. fibrin matrices) that regulates angiogenic events in postnatal life.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.