ArticlePloS one2013
Development and validation of a microarray for the investigation of the CAZymes encoded by the human gut microbiome.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 70 citations in OpenAlex.
- Lactiplantibacillus plantarum P9 for chronic diarrhea in young adults: a large double-blind, randomized, placebo-controlled trial.Nature communications · 2024Trial
- Trial
- Captivity Reduces Diversity and Shifts Composition of the Great Bustard (Ecology and evolution · 2025Article
- Postbiotics from Saccharomyces cerevisiae fermentation stabilize microbiota in rumen liquid digesta during grain-based subacute ruminal acidosis (SARA) in lactating dairy cows.Journal of animal science and biotechnology · 2024Article
- Retraction: Development and Validation of a Microarray for the Investigation of the CAZymes Encoded by the Human Gut Microbiome.PloS one · 2024Article
- Article
- Species variations in the gut microbiota of captive snub-nosed monkeys.Frontiers in endocrinology · 2023Article
- Diversity and functional analysis of rumen and fecal microbial communities associated with dietary changes in crossbreed dairy cattle.PloS one · 2023Article
- Exploring Bacterial Attributes That Underpin Symbiont Life in the Monogastric Gut.Applied and environmental microbiology · 2022Review
- In silico evaluation of a targeted metaproteomics strategy for broad screening of cellulolytic enzyme capacities in anaerobic microbiome bioreactors.Biotechnology for biofuels and bioproducts · 2022Article
- Article
- Effect ofFrontiers in microbiology · 2022Article
- Comparative metagenomics analysis reveals how the diet shapes the gut microbiota in several small mammals.Ecology and evolution · 2022Article
- Metatranscriptomic analysis of colonic microbiota's functional response to different dietary fibers in growing pigs.Animal microbiome · 2021Article
- We and herbivores eat endophytes.Microbial biotechnology · 2021Review
- Degradation of complex arabinoxylans by human colonic Bacteroidetes.Nature communications · 2021Article
- Interplay of Good Bacteria and Central Nervous System: Cognitive Aspects and Mechanistic Considerations.Frontiers in neuroscience · 2021Review
- Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa.Molecular and cellular probes · 2020Article
- Saccharomyces cerevisiae fermentation products (SCFP) stabilize the ruminal microbiota of lactating dairy cows during periods of a depressed rumen pH.BMC veterinary research · 2020Article
- Prebiotics from Seaweeds: An Ocean of Opportunity?Marine drugs · 2019Review
Corrections and comments
- Retraction · 2024-10-14Concerns/Issues about Data · Concerns/Issues about Human Subject Welfare · Conflict of Interest · Investigation by Company/Institution · Investigation by Journal/Publisher · Objections by Author(s) · Upgrade/Update of Prior Notice(s) ·
- Expression of concern · 2022-12-13Concerns/Issues about Human Subject Welfare · Investigation by Journal/Publisher · Updated to Retraction ·
- Expression of concern
- Retracted
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Distal gut bacteria play a pivotal role in the digestion of dietary polysaccharides by producing a large number of carbohydrate-active enzymes (CAZymes) that the host otherwise does not produce. We report here the design of a custom microarray that we used to spot non-redundant DNA probes for more than 6,500 genes encoding glycoside hydrolases and lyases selected from 174 reference genomes from distal gut bacteria. The custom microarray was tested and validated by the hybridization of bacterial DNA extracted from the stool samples of lean, obese and anorexic individuals. Our results suggest that a microarray-based study can detect genes from low-abundance bacteria better than metagenomic-based studies. A striking example was the finding that a gene encoding a GH6-family cellulase was present in all subjects examined, whereas metagenomic studies have consistently failed to detect this gene in both human and animal gut microbiomes. In addition, an examination of eight stool samples allowed the identification of a corresponding CAZome core containing 46 families of glycoside hydrolases and polysaccharide lyases, which suggests the functional stability of the gut microbiota despite large taxonomical variations between individuals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.