Evidence map›Paper›PMID 24391864›Full record

ArticlePloS one2013

SNAP-tag technology optimized for use in Entamoeba histolytica.

Adam Sateriale, Nathan H Roy, Christopher D Huston

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Adam SaterialeUniversity of Vermont Cellular, Molecular, and Biomedical Sciences Program, Burlington, Vermont, United States of America ; University of Vermont Department of Medicine, Burlington, Vermont, United States of America.
Nathan H RoyUniversity of Vermont Cellular, Molecular, and Biomedical Sciences Program, Burlington, Vermont, United States of America ; University of Vermont Microbiology and Molecular Genetics, Burlington, Vermont, United States of America.
Christopher D HustonUniversity of Vermont Cellular, Molecular, and Biomedical Sciences Program, Burlington, Vermont, United States of America ; University of Vermont Microbiology and Molecular Genetics, Burlington, Vermont, United States of America ; University of Vermont Department of Medicine, Burlington, Vermont, United States of America.

Funding

Vermont Immunobioloigy/ Infectious Diseases CenterP20GM103496 · NIGMS · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI BUDD, RALPH C · 2012 to 2015
$8.7M
Vermont Immunology/Infectious Diseases Training GrantT32AI055402 · NIAID · UNIVERSITY OF VERMONT &ST AGRIC COLLEGE · PI GARY E WARD · 2005 to 2026
$2.7M
Molecular regulation of HIV-1 assembly, release and cell-to-cell transmissionR01AI080302 · NIAID · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI THALI, MARKUS · 2009 to 2013
$1.9M
Molecular Mechanism of Entamoeba histolytica phagocytosisR01AI072021 · NIAID · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI HUSTON, CHRISTOPHER D · 2008 to 2012
$1.6M
NIAID NIH HHS R01 AI072021NIAID NIH HHS R01 AI080302NIAID NIH HHS T32 AI055402NIGMS NIH HHS P20 GM103496PHS HHS NIAID R01 A1072021
6 · The paper itself

Abstract

Entamoeba histolytica is a protozoan parasite responsible for invasive intestinal and extraintestinal amebiasis. The pathology of amebiasis is still poorly understood, which can be largely attributed to lack of molecular tools. Here we present the optimization of SNAP-tag technology via codon optimization specific for E. histolytica. The resultant SNAP protein is highly expressed in amebic trophozoites, and shows proper localization when tagged with an endoplasmic reticulum retention signal. We further demonstrate the capabilities of this system using super resolution microscopy, done for the first time in E. histolytica.

Indexed as

Molecular ProbesAnimalsBlotting, WesternCells, CulturedEndoplasmic ReticulumEntamoeba histolyticaEntamoebiasisFlow CytometryGene Expression RegulationMembrane ProteinsMolecular ImagingMembrane ProteinsMolecular Probes

Identifiers

PMID24391864
PMCPMC3877135

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.