ArticlePloS one2013
Identification and characterization of sulfated carbohydrate-binding protein from Lactobacillus reuteri.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Comprehensive Evaluation of Safety and Probiotic Potential of Lactiplantibacillus plantarum HY7715 Isolated from Korean kimchi.Probiotics and antimicrobial proteins · 2026Article
- Foodborne Lactic Acid Bacteria Inactivate Planktonic and SessileFoods (Basel, Switzerland) · 2025Article
- Sharing of Moonlighting Proteins Mediates the Symbiotic Relationship among Intestinal Commensals.Applied and environmental microbiology · 2023Article
- The Mucus Binding Factor Is Not Necessary forInternational journal of molecular sciences · 2022Article
- Complete Genome Sequence of Lactococcus cremoris Strain 7-1, a Lactic Acid Bacterium Isolated from a Traditional Mongolian Milk Product Possessing Mucin-Adhesive Ability.Microbiology resource announcements · 2022Article
- The probiotic and immunomodulation effects ofFrontiers in cellular and infection microbiology · 2022Article
- Adhesion and Colonization of theFrontiers in microbiology · 2022Article
- Molecular Responses of Lactobacilli to Plant Phenolic Compounds: A Comparative Review of the Mechanisms Involved.Antioxidants (Basel, Switzerland) · 2021Review
- Potential ProbioticFrontiers in microbiology · 2021Article
- Glyceraldehyde-3-Phosphate Dehydrogenase Increases the Adhesion ofInternational journal of molecular sciences · 2020Article
- Lactobacillus Cell Surface Proteins Involved in Interaction with Mucus and Extracellular Matrix Components.Current microbiology · 2020Review
- Extracellular Vesicles Produced by Bifidobacterium longum Export Mucin-Binding Proteins.Applied and environmental microbiology · 2020Article
- A new approach for analyzing an adhesive bacterial protein in the mouse gastrointestinal tract using optical tissue clearing.Scientific reports · 2019Article
- 3-Sulfogalactosyl-dependent adhesion ofThe Journal of biological chemistry · 2017Article
- Article
- Elongation factor Tu is a multifunctional and processed moonlighting protein.Scientific reports · 2017Article
- Gallibacterium elongation factor-Tu possesses amyloid-like protein characteristics, participates in cell adhesion, and is present in biofilms.Journal of microbiology (Seoul, Korea) · 2017Article
- Use of Atomic Force Microscopy to Study the Multi-Modular Interaction of Bacterial Adhesins to Mucins.International journal of molecular sciences · 2016Article
- Adhesion Properties of Lactic Acid Bacteria on Intestinal Mucin.Microorganisms · 2016Review
- Isolation of lactic acid bacteria bound to the porcine intestinal mucosa and an analysis of their moonlighting adhesins.Bioscience of microbiota, food and health · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously purified a putative sulfated-galactosylceramide (sulfatide)-binding protein with a molecular weight of 47 kDa from the cell surface of Lactobacillus reuteri JCM1081. The aim of this study was to identify the 47-kDa protein, examine its binding to sulfated glycolipids and mucins, and evaluate its role in bacterial adhesion to mucosal surfaces. By cloning and sequencing analysis, the 47-kDa protein was identified as elongation factor-Tu (EF-Tu). Adhesion properties were examined using 6 × Histidine-fused EF-Tu (His6-EF-Tu). Surface plasmon resonance analysis demonstrated pH-dependent binding of His6-EF-Tu to sulfated glycolipids, but not to neutral or sialylated glycolipids, suggesting that a sulfated galactose residue was responsible for EF-Tu binding. Furthermore, His6-EF-Tu was found to bind to porcine gastric mucin (PGM) by enzyme-linked immunosorbent assay. Binding was markedly reduced by sulfatase treatment of PGM and in the presence of acidic and desialylated oligosaccharide fractions containing sulfated carbohydrate residues prepared from PGM, demonstrating that sulfated carbohydrate moieties mediated binding. Histochemical staining revealed similar localization of His6-EF-Tu and high iron diamine staining in porcine mucosa. These results indicated that EF-Tu bound PGM via sulfated carbohydrate moieties. To characterize the contribution of EF-Tu to the interaction between bacterial cells and PGM, we tested whether anti-EF-Tu antibodies could inhibit the interaction. Binding of L. reuteri JCM1081 to PGM was significantly blocked in a concentration-dependent matter, demonstrating the involvement of EF-Tu in bacterial adhesion. In conclusion, the present results demonstrated, for the first time, that EF-Tu bound sulfated carbohydrate moieties of sulfated glycolipids and sulfomucin, thereby promoting adhesion of L. reuteri to mucosal surfaces.
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