Evidence map›Paper›PMID 24391804›Full record

ArticlePloS one2013

Light-dependent and circadian transcription dynamics in vivo recorded with a destabilized luciferase reporter in Neurospora.

François Cesbron, Michael Brunner, Axel C R Diernfellner

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 35 citations in OpenAlex.

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  6. Antisense Transcription of theJournal of biological rhythms · 2023
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  9. Casein kinase 1 and disordered clock proteins form functionally equivalent, phospho-based circadian modules in fungi and mammals.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  10. Cell-Type-Specific Circadian Bioluminescence Rhythms inJournal of biological rhythms · 2022
    Article
  11. Article
  12. A pathway linking translation stress to checkpoint kinase 2 signaling inProceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  13. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

François CesbronUniversity of Heidelberg Biochemistry Center (BZH), Im Neuenheimer Feld, Heidelberg, Germany.
Michael BrunnerUniversity of Heidelberg Biochemistry Center (BZH), Im Neuenheimer Feld, Heidelberg, Germany.
Axel C R DiernfellnerUniversity of Heidelberg Biochemistry Center (BZH), Im Neuenheimer Feld, Heidelberg, Germany.
Heidelberg University · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We show that firefly luciferase is a stable protein when expressed at 25 °C in Neurospora, which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription dynamics associated with the Neurospora circadian clock and its blue-light photosensory system over the course of several days. We show that the tool is suitable to faithfully monitor rapid, but also subtle changes in transcription in a medium to high throughput format.

Indexed as

LightTranscription, GeneticAmino Acid SequenceCircadian RhythmFungal ProteinsLuciferasesLuminescent MeasurementsMolecular Sequence DataNeurosporaPolymerase Chain ReactionFRQ protein, Neurospora crassaFungal ProteinsLuciferases

Identifiers

PMID24391804
PMCPMC3877077
OpenAlexW2016113156

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.