ArticlePloS one2013
Assessment of the geographic origins of pinewood nematode isolates via single nucleotide polymorphism in effector genes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Whole-Genome Sequences of 13 Chinese Indigenous Pinewood Nematodes,International journal of molecular sciences · 2024Article
- Pine wilt disease: what do we know from proteomics?BMC plant biology · 2024Review
- Genetic diversity and genome-wide association analysis of pine wood nematode populations in different regions of China.Frontiers in plant science · 2023Article
- Comprehensive Analysis of Copy Number Variations on Glycoside Hydrolase 45 Genes among DifferentInternational journal of molecular sciences · 2022Article
- Population differentiation and epidemic tracking of Bursaphelenchus xylophilus in China based on chromosome-level assembly and whole-genome sequencing data.Pest management science · 2022Article
- Morphometric and Molecular Diversity among Seven European Isolates ofPlants (Basel, Switzerland) · 2021Article
- Molecular variation among virulent and avirulent strains of the quarantine nematode Bursaphelenchus xylophilus.Molecular genetics and genomics : MGG · 2021Article
- Virulence Biomarkers ofFrontiers in plant science · 2021Article
- Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis.Scientific reports · 2016Article
- Genome-wide variation in the pinewood nematode Bursaphelenchus xylophilus and its relationship with pathogenic traits.BMC genomics · 2015Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pinewood nematode, Bursaphelenchus xylophilus, is native to North America but it only causes damaging pine wilt disease in those regions of the world where it has been introduced. The accurate detection of the species and its dispersal routes are thus essential to define effective control measures. The main goals of this study were to analyse the genetic diversity among B. xylophilus isolates from different geographic locations and identify single nucleotide polymorphism (SNPs) markers for geographic origin, through a comparative transcriptomic approach. The transcriptomes of seven B. xylophilus isolates, from Continental Portugal (4), China (1), Japan (1) and USA (1), were sequenced in the next generation platform Roche 454. Analysis of effector gene transcripts revealed inter-isolate nucleotide diversity that was validated by Sanger sequencing in the genomic DNA of the seven isolates and eight additional isolates from different geographic locations: Madeira Island (2), China (1), USA (1), Japan (2) and South Korea (2). The analysis identified 136 polymorphic positions in 10 effector transcripts. Pairwise comparison of the 136 SNPs through Neighbor-Joining and the Maximum Likelihood methods and 5-mer frequency analysis with the alignment-independent bilinear multivariate modelling approach correlated the SNPs with the isolates geographic origin. Furthermore, the SNP analysis indicated a closer proximity of the Portuguese isolates to the Korean and Chinese isolates than to the Japanese or American isolates. Each geographic cluster carried exclusive alleles that can be used as SNP markers for B. xylophilus isolate identification.
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