ArticleFrontiers in immunology2013
Preparing unbiased T-cell receptor and antibody cDNA libraries for the deep next generation sequencing profiling.
Article in Frontiers in immunology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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Who cites it
96 citing papers in PubMed.
- Cytotoxic CD4+ T lymphocytes may induce endothelial cell apoptosis in systemic sclerosis.The Journal of clinical investigation · 2020Trial
- Recovering CDR3 sequences from RNA-seq data: a critical assessment of computational and experimental strategies.Genomics & informatics · 2026Review
- The case for caution in the application of whole-exome sequencing data for immune repertoire analysis.Briefings in bioinformatics · 2026Article
- High expression of the underexplored SLC4A11 protein-coding transcript is specific to the corneal endothelium.Scientific reports · 2026Article
- Profiling adaptive immunity: A quantitative framework for immune repertoire dynamics and clinical diagnostics.Fundamental research · 2026Article
- Identification of Sjögren's disease-associated T cell receptor motifs through deep sequencing.JCI insight · 2025Article
- Plasmodium falciparum infection induces T cell tolerance that is associated with decreased disease severity upon re-infection.The Journal of experimental medicine · 2025Article
- Evaluation of T-cell repertoire by flow cytometric analysis in primary immunodeficiencies with DNA repair defects.Scandinavian journal of immunology · 2025Article
- T-cell Receptor Repertoire Analysis in the Context of Transarterial Chemoembolization Synergy with Systemic Therapy for Hepatocellular Carcinoma.Journal of clinical and translational hepatology · 2025Review
- Tick-borne encephalitis virus variants drive distinct TCR repertoire alterations.Frontiers in immunology · 2025Article
- Restoration of T and B Cell Differentiation after RAG1 Gene Transfer in Human RAG1 Defective Hematopoietic Stem Cells.Biomedicines · 2024Article
- Donor regulatory T cells rapidly adapt to recipient tissues to control murine acute graft-versus-host disease.Nature communications · 2024Article
- Development of a next-generation sequencing protocol for the canine T cell receptor beta chain repertoire.Veterinary immunology and immunopathology · 2024Article
- Unraveling the chicken T cell repertoire with enhanced genome annotation.Frontiers in immunology · 2024Article
- Detecting T-cell clonal expansions and quantifying clone survival using deep profiling of immune repertoires.Frontiers in immunology · 2024Article
- EBV T-cell immunotherapy generated by peptide selection has enhanced effector functionality compared to LCL stimulation.Frontiers in immunology · 2024Article
- Chicken γδ T cells proliferate upon IL-2 and IL-12 treatment and show a restricted receptor repertoire in cell culture.Frontiers in immunology · 2024Article
- T-Cell Receptor Repertoire Sequencing in the Era of Cancer Immunotherapy.Clinical cancer research : an official journal of the American Association for Cancer Research · 2023Article
- Transgenic HA-1-Specific CD8Cancers · 2023Article
- Article
36 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput sequencing has the power to reveal the nature of adaptive immunity as represented by the full complexity of T-cell receptor (TCR) and antibody (IG) repertoires, but is at present severely compromised by the quantitative bias, bottlenecks, and accumulated errors that inevitably occur in the course of library preparation and sequencing. Here we report an optimized protocol for the unbiased preparation of TCR and IG cDNA libraries for high-throughput sequencing, starting from thousands or millions of live cells in an investigated sample. Critical points to control are revealed, along with tips that allow researchers to minimize quantitative bias, accumulated errors, and cross-sample contamination at each stage, and to enhance the subsequent bioinformatic analysis. The protocol is simple, reliable, and can be performed in 1-2 days.
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