Evidence map›Paper›PMID 24390841›Full record

ArticleJournal of microbiology (Seoul, Korea)2014

Surface display of the HPV L1 capsid protein by the autotransporter Shigella IcsA.

Dan Xu, Xiaofeng Yang, Depu Wang, Jun Yu, Yili Wang

Abstract read
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In one paragraph

Article in Journal of microbiology (Seoul, Korea), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Dan XuInstitute of Cancer Research, School of Life Sciences and Technology, Xi'an Jiaotong University, Xi'an, P. R. China.
Xiaofeng Yang
Depu Wang
Jun Yu
Yili Wang

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Autotransporters have become attractive tools for surface expression of foreign proteins in Gram-negative bacteria. In this study, the Shigella autotransporter IcsA, has been exploited to express the human papillomavirus (HPV) type 16 L1 capsid protein in Shigella sonnei and Escherichia coli. The L1 gene was fused in-frame to replace the coding sequence of the IcsA passenger domain that is responsible for actin-based motility. The resultant hybrid protein could be detected by an anti-L1 antibody on the surface of S. sonnei and E. coli. In E. coli, the protein was expressed on the entire surface of the bacterium. In contrast, the protein was detected mainly at one pole of the Shigella bacterium. However, the protein became evenly distributed on the surface of the Shigella bacterium when the icsP gene was removed. Our study demonstrated the possibility of exploiting autotransporters for surface expression of large, heterologous viral proteins, which may be a useful strategy for vaccine development.

Indexed as

Cell Surface Display TechniquesBacterial ProteinsCapsid ProteinsDNA-Binding ProteinsEscherichia coliMembrane ProteinsOncogene Proteins, ViralRecombinant Fusion ProteinsShigella sonneiTranscription FactorsBacterial ProteinsCapsid ProteinsDNA-Binding ProteinsHPV L1 protein, Human papillomavirusMembrane ProteinsOncogene Proteins, ViralRecombinant Fusion ProteinsTranscription FactorsvirG protein, Shigella flexneri

Identifiers

PMID24390841

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.